Metabolomic profiling of the astaxanthin accumulation process induced by high light in Haematococcus pluvialis
Metabolomic profiling of the astaxanthin accumulation process induced by high light in Haematococcus pluvialis
复制标题
高光诱导雨生红球藻虾青素积累过程的代谢组学分析
DOI：
10.1016/j.algal.2016.09.019
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发表时间：
2016-12-01
影响因子：
5.1
通讯作者：
Jia, Shiru
中科院分区：
文献类型：
--
作者：
Lv, Hexin;Xia, Feng;Jia, Shiru
在强光照射下，雨红球菌的生长分为三个色素沉着期：绿色期、黄色期和红色期。分类依据虾青素和叶绿素含量以及细胞形态。对三个色素沉着阶段进行代谢分析。采用气相色谱-质谱联用技术对81种代谢物进行了鉴定和定量，包括23种氨基酸、19种糖、15种有机酸、8种醇、7种胺、4种核酸和5种其他代谢物。根据三个阶段的变化情况，将其分为四组。通过主成分分析、偏最小二乘分析和分层聚类分析，发现了潜在的生物标志物，揭示了细胞代谢途径的细节。黄期部分细胞保护代谢产物如蔗糖、脯氨酸、谷氨酸含量增加。这些代谢物的前体是卡尔文循环和TCA循环的中间产物，表明这两个循环为细胞保护代谢物的合成提供了更多的前体。提出了一种假定的强光下雨杉代谢调节模型。本研究首次从代谢组学角度研究了强光诱导雨水杨虾青素积累过程。(C) 2016 Elsevier B.V.版权所有
The growth of Haematococcus pluvialis exposed to high light was divided into three pigmentation phases: a green phase, a yellow phase and a red phase. Classification was based on astaxanthin and chlorophyll content and cell morphology. Metabolic profiling of the three pigmentation phases was performed. A total of 81 metabolites were identified and quantified by gas chromatography-mass spectrometry, including 23 amino acids, 19 sugars, 15 organic acids, 8 alcohols, 7 amines, 4 nucleic acids and 5 others. These were subdivided into four groups according to their changes during the three phases. The clustering of metabolites was revealed, and potential biomarkers were identified by principal component analysis, partial least squares analysis and hierarchical clustering analysis, suggesting details of the metabolic pathways of cells. The contents of some cytoprotective metabolites were increased in the yellow phase, such as sucrose, proline and glutamic acid. The precursors of these metabolites are the intermediates of the Calvin cycle and the TCA cycle, indicated those two cycles provided more precursors for the synthesis of the cytoprotective metabolites. A hypothetical metabolic regulation model of H. pluvialis exposed to high light was proposed. Our study provides the first metabolomics view of the astaxanthin accumulation process that is induced by high light in H. pluvialis. (C) 2016 Elsevier B.V. All rights reserved.