Circulating microRNA profiles based on direct S-Poly(T)Plus assay for detection of coronary heart disease
Circulating microRNA profiles based on direct S-Poly(T)Plus assay for detection of coronary heart disease
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基于直接 S-Poly(T)Plus 检测的循环 microRNA 谱用于检测冠心病
DOI：
10.1111/jcmm.15001
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发表时间：
2020-04-28
影响因子：
5.3
通讯作者：
Gou，Deming
中科院分区：
文献类型：
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作者：
Su，Mingyang;Niu，Yanqin;Gou，Deming
冠心病（CHD）是全球心脏相关死亡的主要原因之一。传统的诊断技术是无效的，不足以诊断冠心病的准确性更高。为了使用循环microRNA（miRNAs）作为诊断CHD的非侵入性、特异性和敏感性生物标志物，203名CHD患者和144名年龄匹配的对照（126名高危对照和18名健康志愿者）入组本研究。采用直接S‐Poly（T）Plus方法鉴定冠心病患者的新miRNAs表达谱，并评价其临床诊断价值。该方法是一种无需RNA提取的稳健定量方法，简化了程序，减少了变异，特别是提高了准确度。选择CHD患者和高风险对照之间的12种差异表达的miRNA，并在具有96个血浆样本的验证集1中评估其性能。最后，6在验证集2中验证了这12种miRNAs（miR-15 b-5 p、miR-29 c-3 p、miR-199 a-3 p、miR-320 e、miR-361 - 5 p和miR-378 b），灵敏度为92.8%，特异性为89.5%，AUC为0.971（95%置信区间，0.948 - 0.993，P < .001）。血浆分离表明，只有少量的miRNA组装成EV。直接S-Poly（T）Plus方法可用于疾病诊断，12种独特的miRNAs可用于冠心病的诊断。
Coronary heart disease (CHD) is one of the leading causes of heart‐associated deaths worldwide. Conventional diagnostic techniques are ineffective and insufficient to diagnose CHD with higher accuracy. To use the circulating microRNAs (miRNAs) as non‐invasive, specific and sensitive biomarkers for diagnosing of CHD, 203 patients with CHD and 144 age‐matched controls (126 high‐risk controls and 18 healthy volunteers) were enrolled in this study. The direct S‐Poly(T)Plus method was used to identify novel miRNAs expression profile of CHD patients and to evaluate their clinical diagnostic value. This method is an RNA extraction‐free and robust quantification method, which simplifies procedures, reduces variations, in particular increases the accuracy. Twelve differentially expressed miRNAs between CHD patients and high‐risk controls were selected, and their performances were evaluated in validation set‐1 with 96 plasma samples. Finally, six (miR‐15b‐5p, miR‐29c‐3p, miR‐199a‐3p, miR‐320e, miR‐361‐5p and miR‐378b) of these 12 miRNAs were verified in validation set‐2 with a sensitivity of 92.8% and a specificity of 89.5%, and the AUC was 0.971 (95% confidence interval, 0.948‐0.993,P< .001) in a large cohort for CHD patients diagnosis. Plasma fractionation indicated that only a small amount of miRNAs were assembled into EVs. Direct S‐Poly(T)Plus method could be used for disease diagnosis and 12 unique miRNAs could be used for diagnosis of CHD.