Antiproliferative effect of curcumin (diferuloylmethane) against human breast tumor cell lines
Antiproliferative effect of curcumin (diferuloylmethane) against human breast tumor cell lines
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DOI：
10.1097/00001813-199706000-00010
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发表时间：
1997-06-01
影响因子：
2.3
通讯作者：
Aggarwal, BB
中科院分区：
文献类型：
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作者：
Mehta, K;Pantazis, P;Aggarwal, BB
可以抑制肿瘤细胞增殖的药理学安全化合物具有作为抗癌药物的潜力。姜黄素是一种二阿魏酰甲烷，是食用香料姜黄 (Curcuma longa) 的主要活性成分，在体内具有抗癌特性，在体外，它可抑制 c-jun/Ap-1 和 NF-kappa B 激活以及 1 型人类免疫缺陷病毒长末端重复定向基因表达。我们研究了姜黄素对几种乳腺肿瘤细胞系的抗增殖作用，包括激素依赖性和非依赖性以及多重耐药（MDR）细胞系。通过[H-3]胸苷掺入、台盼蓝排除、结晶紫染料摄取和流式细胞术监测细胞生长抑制。所有测试的细胞系，包括MDR阳性细胞系，对姜黄素高度敏感。姜黄素的生长抑制作用具有时间和剂量依赖性，并与其对鸟氨酸脱羧酶活性的抑制相关。姜黄素优先将细胞阻滞在细胞周期的 G(2)/S 期。姜黄素诱导的细胞死亡既不是由于细胞凋亡，也不是由于细胞凋亡相关基因（包括 Bcl-2、p53、细胞周期蛋白 a 和转谷氨酰胺酶）表达的任何显着变化。总的来说，我们的结果表明姜黄素是乳腺肿瘤细胞的有效抗增殖剂，并且可能具有作为抗癌剂的潜力。
Pharmacologically safe compounds that can inhibit the proliferation of tumor cells have potential as anticancer agents. Curcumin, a diferuloylmethane, is a major active component of the food flavor turmeric (Curcuma longa) that exhibits anticarcinogenic properties in vivo, In vitro, it suppressed c-jun/Ap-1 and NF-kappa B activation and type 1 human immunodeficiency virus long-terminal repeat-directed gene expression. We examined the antiproliferative effects of curcumin against several breast tumor cell lines, including hormone-dependent and -independent and multidrug-resistant (MDR) lines. Cell growth inhibition was monitored by [H-3]thymidine incorporation, Trypan blue exclusion, crystal violet dye uptake and flow cytometry, All the cell lines tested, including the MDR-positive ones, were highly sensitive to curcumin, The growth inhibitory effect of curcumin was time- and dose-dependent, and correlated with its inhibition of ornithine decarboxylase activity. Curcumin preferentially arrested cells in the G(2)/S phase of the cell cycle. Curcumin-induced cell death was neither due to apoptosis nor to any significant change in the expression of apoptosis-related genes, including Bcl-2, p53, cyclin a and transglutaminase, Overall our results suggest that curcumin is a potent antiproliferative agent for breast tumor cells and may have potential as an anticancer agent.