Plasmodium falciparum: Allelic variation in the merozoite surface protein 1 gene in wild isolates from southern Vietnam
Plasmodium falciparum: Allelic variation in the merozoite surface protein 1 gene in wild isolates from southern Vietnam
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DOI：
10.1006/expr.1997.4147
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发表时间：
1997-05-01
影响因子：
2.1
通讯作者：
Tanabe, K
中科院分区：
文献类型：
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作者：
Kaneko, O;Kimura, M;Tanabe, K
恶性疟原虫裂殖子表面蛋白 1 (MSP1) 基因中的等位基因变异表达为可变块中等位基因类型的关联。在本研究中，使用可检测 24 种不同 MSP1 关联类型的 PCR 策略来研究 MSP1 基因的等位基因变异。我们在从越南南部收集的 136 个野生分离株中鉴定了 236 个不同的关联类型克隆，分析表明：（1）MSPI 基因中心部分的两个代表性等位基因类型之间不存在重组，（2）MSP1 关联类型的频率分布在不同人群中没有差异，（3）特定的 MSP1 关联类型占主导地位。对等位基因类型关联的统计分析表明，块 4 和 6 之间存在显着的非随机关联，但块 2 和 4 以及 2 和 6 之间则不然。这些结果表明，选择有利于特定的 MSPI 关联类型。此外，对 31 个分离株的直接测序证实了 MSPI C 端 19-kDa Cys 丰富区域的序列替换，支持了该区域有限变异的概念，这是一种强大的疫苗候选分子。 (C) 1997 年学术出版社。
Allelic variation in the Plasmodium falciparum merozoite surface protein 1 (MSP1) gene is expressed as an association of allelic types in variable blocks. In this study, a PCR strategy that can detect 24 different MSP1 association types was used to investigate allelic Variation in the MSP1 gene. We identified 236 distinct association type clones in 136 wild isolates collected from southern Vietnam, analysis of which revealed that (1) recombination between two representative allelic types in the central part of the MSPI gene did not exist, (2) frequency distribution of MSP1 association types did not differ indifferent population groups, and (3) particular MSP1 association types were predominant. Statistical analysis for the association of allelic types indicated significant, nonrandom associations between blocks 4 and 6 but not between blocks 2 and 4, and 2 and 6. These results suggest that selection operates in favor of particular MSPI association types. In addition, direct sequencing of 31 isolates confirmed reported sequence substitutions in the C-terminal 19-kDa Cys-rich region of MSPI, supporting a notion of limited variations in this region, a strong vaccine candidate molecule. (C) 1997 Academic Press.