Improvement of the Immunomagnetic Separation Method Selective for Escherichia coli O157 Strains
Improvement of the Immunomagnetic Separation Method Selective for Escherichia coli O157 Strains
复制标题
大肠杆菌O157菌株免疫磁珠选择性分离方法的改进
DOI：
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发表时间：
1998
影响因子：
4.4
通讯作者：
Takahiro Tomoyasu
中科院分区：
文献类型：
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作者：
Takahiro Tomoyasu
免疫磁分离是一种有效的富集方法，可对预富集培养的大肠杆菌O157细胞和非O157大肠杆菌细胞进行选择性富集。然而，大肠杆菌细胞被非特异性地吸附在固体表面上。在常规的免疫磁分离方法中，这种非特异性吸附干扰了免疫磁分离。发现这种干扰可以用低离子强度溶液来消除。用该溶液进行免疫磁分离时，大肠杆菌O157细胞与非O157大肠杆菌的比例由常规免疫磁分离法的9.6倍提高到31.4倍。通过使用大肠杆菌157加标样品，成功地评估了该溶液的有效性。
ABSTRACT Immunomagnetic separation is a useful enrichment method selective for Escherichia coli O157 cells against non-O157 E. coli cells from a preenrichment culture. However, E. coli cells are adsorbed onto a solid surface nonspecifically. With the conventional immunomagnetic separation method, this nonspecific adsorption interfered with immunomagnetic separation. It was found that this interference could be reduced with a low-ionic-strength solution. When immunomagnetic separation was carried out with this solution, the proportion of E. coli O157 cells to non-O157 E. coli cells increased from 9.6 to 31.4 times compared to the proportion obtained by the conventional immunomagnetic separation method. The effectiveness of this solution was successfully evaluated by the use of E. coliO157-spiked samples.