Transcription-coupled events associating with immunoglobulin switch region chromatin
Transcription-coupled events associating with immunoglobulin switch region chromatin
复制标题
DOI：
10.1126/science.1092481
复制
发表时间：
2003-12-19
期刊：
影响因子：
56.9
通讯作者：
Shimizu, A
中科院分区：
文献类型：
--
作者：
Nambu, Y;Sugai, M;Shimizu, A
在抗体免疫球蛋白基因座处的类别转换重组（CSR）由种系转录（GLT）偶联模式调节。阳离子的开关区域，其中CSR发生的可及性。在这里，我们表明，组蛋白乙酰化的开关区域与CSR，但组蛋白乙酰化不能单独促进CSR或GLT。活化诱导的胞苷脱氨酶（AID）以GLT偶联的方式特异性地与CSR靶染色质结合，这可能通过AID和转录机制之间的物理相互作用而潜在地发生。这些数据表明GLT在调节染色质可及性中的重要作用，强烈表明AID的靶点是染色质DNA。我们的研究结果提供了对AID的作用和CSR的监管机制的见解。
Class switch recombination (CSR) at the antibody immunoglobulin locus is regulated by germline transcription (GLT) - coupled modi. cations in the accessibility of the switch region, where CSR takes place. Here we show that histone acetylation of switch regions is linked to CSR but that histone acetylation cannot alone promote CSR or GLT. Activation-induced cytidine deaminase (AID) specifically associates with the CSR target chromatin in a GLT-coupled manner, which may occur potentially by means of physical interaction between AID and the transcription machinery. These data indicate an important role of GLT in the regulation of chromatin accessibility, strongly suggesting that the target of AID is chromatin DNA. Our results give insights on the role of AID and the regulatory mechanism of CSR.