Flow cytometry analysis of thymic epithelial cells and their subpopulation
Flow cytometry analysis of thymic epithelial cells and their subpopulation
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胸腺上皮细胞及其亚群的流式细胞术分析
DOI：
10.1007/978-1-4939-2809-5_5
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发表时间：
2016
影响因子：
--
通讯作者：
Takahama Y
中科院分区：
文献类型：
--
作者：
Ohigashi I;Takahama Y
胸腺实质被划分为皮层和髓质，分别由胸腺皮质上皮细胞（cortical胸腺上皮细胞，cTECs）和胸腺髓质上皮细胞（mTECs）构成。cTECs和mTECs本质上不同地调控T细胞的发育和库选择。因此，T细胞发育和选择的生物学除了研究发育中的T细胞和包括树突状细胞在内的其他造血细胞外，还包括对tec的研究。在本章中，我们描述了流式细胞术分析和分类TECs及其亚群的方法，包括cTECs和mTECs。
The parenchyma of the thymus is compartmentalized into the cortex and the medulla, which are constructed by cortical thymic epithelial cells (cortical TECs, cTECs) and medullary thymic epithelial cells (mTECs), respectively. cTECs and mTECs essentially and differentially regulate the development and repertoire selection of T cells. Consequently, the biology of T cell development and selection includes the study of TECs in addition to the study of developing T cells and other hematopoietic cells including dendritic cells. In this chapter, we describe the methods for flow cytometric analysis and sorting of TECs and their subpopulations, including cTECs and mTECs.