Reentry of T cells to the adult thymus is restricted to activated T cells.
Reentry of T cells to the adult thymus is restricted to activated T cells.
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T细胞重新进入成人胸腺，仅限于活化的T细胞。
DOI：
10.1084/jem.173.5.1039
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发表时间：
1991-05-01
期刊：
影响因子：
--
通讯作者：
Sprent J
中科院分区：
文献类型：
--
作者：
Agus DB;Surh CD;Sprent J
为了寻求有关成熟 T 细胞迁移至胸腺的能力的信息，给小鼠注射了富含静息 T 细胞或 T 母细胞的 Thy-1 标记群体；通过胸腺低温切片染色和细胞悬液的 FACS 分析来评估供体细胞在宿主胸腺中的定位。注射纯化的静息T细胞后，即使注射大剂量的细胞，胸腺归巢也极其有限。相比之下，体内产生的T母细胞大量迁移到胸腺。 T 母细胞的胸腺归巢效率比静息 T 细胞高 50 倍以上。母细胞主要集中在髓质中，并在转移后至少 1 个月内保留在胸腺中。有趣的是，受辐射宿主胸腺中 T 母细胞的定位比正常宿主高 10 倍。在注射了与 DNA 前体 125I-5-iodo-2' 脱氧尿苷 (125IDUR) 体外孵育的 T 母细胞的小鼠中，胸腺归巢尤其明显。将 125IDUR 标记的原始细胞转移到受辐射的宿主中，高达 5% 的注射计数位于宿主胸腺中。这些数据表明，T 母细胞的胸腺归巢可能很大程度上限于 S 期细胞。母细胞进入胸腺的生理意义尚不清楚。讨论了这些细胞参与胸腺内耐受诱导的可能性。
To seek information on the capacity of mature T cells to migrate to the thymus, mice were injected with Thy-1-marked populations enriched for resting T cells or T blast cells; localization of the donor cells in the host thymus was assessed by staining cryostat sections of thymus and by FACS analysis of cell suspensions. With injection of purified resting T cells, thymic homing was extremely limited, even with injection of large doses of cells. By contrast, in vivo generated T blast cells migrated to the thymus in substantial numbers. Thymic homing by T blasts was greater than 50-fold more efficient than with resting T cells. Blast cells localized largely in the medulla and remained in the thymus for at least 1 mo post-transfer. Interestingly, localization of T blasts in the thymus was 10-fold higher in irradiated hosts than normal hosts. Thymic homing was especially prominent in mice injected with T blasts incubated in vitro with the DNA precursor, 125I- 5-iodo-2'deoxyuridine (125IDUR); with transfer of 125IDUR-labeled blasts to irradiated hosts, up to 5% of the injected counts localized in the host thymus. These data suggest that thymic homing by T blasts might be largely restricted to cells in S phase. The physiological significance of blast cell entry to the thymus is unclear. The possibility that these cells participate in intrathymic tolerance induction is discussed.