FLUORESCENT LATEX MICROSPHERES AS A RETROGRADE TRACER IN THE PERIPHERAL NERVOUS-SYSTEM
FLUORESCENT LATEX MICROSPHERES AS A RETROGRADE TRACER IN THE PERIPHERAL NERVOUS-SYSTEM
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DOI：
10.1016/0006-8993(89)90520-9
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发表时间：
1989-05-08
期刊：
影响因子：
2.9
通讯作者：
FOOTE, WE
中科院分区：
文献类型：
--
作者：
COLIN, W;DONOFF, RB;FOOTE, WE
罗丹明标记的乳胶微球被用作周围神经系统的荧光逆行示踪剂。将微球应用于挤压或完整的下牙槽神经后，对兔三叉神经节的检查显示：（1）微球仅被受损的轴突吸收：（2）微球在三叉神经细胞体中保留长达3个月，而没有降解或扩散到细胞外结构;（3）含有微球的细胞能够再生轴突，如通过诱发的感觉动作前足的返回和True blue的逆行轴突运输所证明的。因此，荧光微球可能是有用的工具，在周围神经系统再生和发育的体内存活研究。
Rhodamine labeled latex microspheres were used as a fluorescent retrograde tracer in the peripheral nervous system. Examination of rabbit trigeminal ganglia following application of microspheres to crushed or intact inferior alveolar nerve revealed that: (1) microspheres were taken up by only damaged axons; (2) microspheres remained in trigeminal cells bodies for up to 3 months without degradation or diffusion to extracellular structures; and (3) cells containing microspheres were capable of regenerating axons as evidenced by the return of evoked sensory action ptoentials and the retrograde axonal transport of True blue. Thus, fluorescent microspheres may be useful tools for in vivo survival studies of peripheral nervous system regeneration and development.