Characterisation of fractalkine/CX3CL1 and fractalkine receptor (CX3CR1) expression in abdominal aortic aneurysm disease
Characterisation of fractalkine/CX3CL1 and fractalkine receptor (CX3CR1) expression in abdominal aortic aneurysm disease
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DOI：
10.1016/j.ejvs.2008.01.014
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发表时间：
2008-07-01
影响因子：
5.7
通讯作者：
Carding, S. R.
中科院分区：
文献类型：
--
作者：
Patel, A.;Jagadesham, V. P.;Carding, S. R.
目的：Fractalkine （CX3CL1）通过与表达在CD56(+)/CD16(+) NK细胞和CD8(+) T细胞上的Fractalkine受体（CX3CR1）相互作用，促进白细胞的粘附和外溢。本研究旨在验证CX3CL1-CX3CR1相互作用参与AAA组织炎症浸润的假设。设计与方法：采用免疫组化（IHC）方法检测CX3CR1在AAA组织中的表达。采用多参数流式细胞术（FC）检测AAA患者及健康人AAA组织及外周血t细胞（CD3(+)）和NK细胞（CD56(+)） CX3CR1的表达。体外原代细胞培养检测血管内皮细胞（vEC）和平滑肌细胞（vSMC）对CX3CL1表达的调控。结果：CX3CR1（+）细胞在19/28 AAA组织样本中检测到，且主要定位于外膜。与健康对照相比，AAA患者外周血中CX3CR1(+) NK细胞（60.0-88.6%）和T细胞（7.5-39.4%）的百分比更高。此外，炎症性AAA组织中CX3CR1(+) NK细胞（91%）和T细胞（94%）的频率高于动脉粥样硬化性AAA组织。促炎细胞因子TNF - α增加vSMC和vEC中fractalkine的表达。结论：在AAA疾病中存在CX3CL1（+）和CX3CR1（+）细胞，它们的相互作用可能有助于AAA组织中炎症细胞的募集。(C) 2008年欧洲血管外科学会。Elsevier Ltd.出版。版权所有。
Objectives: Fractalkine (CX3CL1) promotes adhesion and extravasation of leucocytes through interactions with fractalkine receptor (CX3CR1) expressed on CD56(+)/CD16(+) NK cells and CD8(+) T cells. The current study aims to test the hypothesis the CX3CL1-CX3CR1 interaction contributes to the inflammatory infiltrate in AAA tissue.Design and methods: Immunohistochemistry (IHC) was used to define expression of CX3CR1 in AAA tissue. Multi-parametric flow cytometry (FC) was used to determine CX3CR1 expression on T-cells (CD3(+)) and NK cells (CD56(+)) from AAA tissue and peripheral blood of AAA patients and healthy controls. Regulation of CX3CL1 expression by vascular endothelial (vEC) and smooth muscle cells (vSMC) was examined in vitro using primary cell cultures.Results: CX3CR1(+) cells were detected in 19/28 AAA tissue samples and predominately localised in the adventitia. PBMCs from patients with AAA demonstrated higher percentages of CX3CR1(+) NK cells (60.0-88.6%) and T cells (7.5-39.4%) compared with healthy controls. Furthermore, the frequency of CX3CR1(+) NK cells (91%) and T cells (94%) in inflammatory AAA tissue were higher than in atherosclerotic AAA tissue. The pro-inflammatory cytokine TNF alpha increased expression of fractalkine by vSMC and vEC.Conclusion: CX3CL1(+) and CX3CR1(+) cells are present in AAA disease and their interaction may contribute to the recruitment of inflammatory cells seen in AAA tissue. (C) 2008 European Society for Vascular Surgery. Published by Elsevier Ltd. All rights reserved.