PIG SKIN AS TEST SUBSTRATE FOR EVALUATING TOPICAL ANTIMICROBIAL ACTIVITY
PIG SKIN AS TEST SUBSTRATE FOR EVALUATING TOPICAL ANTIMICROBIAL ACTIVITY
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DOI：
10.1128/jcm.24.3.343-348.1986
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发表时间：
1986-09-01
影响因子：
9.4
通讯作者：
WHITE, JH
中科院分区：
文献类型：
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作者：
BUSH, LW;BENSON, LM;WHITE, JH
证明了用污染病原菌的猪皮作为皮肤消毒剂评价的模型基质的可行性。描述了一种测试方法，该方法安全、使用方便，并适用于各种洗手条件。治疗方案、病原体污染条件和应用技术变量都可以被仔细控制以模拟临床使用条件，并且应用技术变量都可以被仔细控制以模拟临床使用条件。将通过接触转移的微生物数量与猪皮上的总微生物计数进行比较。根据污染条件的性质，转移的微生物数量为总微生物的10%至60%。多次压印和剥离测量的累积结果与接种微生物的浓度一致。用酒精溶液进行的试验验证了该方法，并清楚地表明了局部抗微生物活性对酒精浓度和结构的依赖性。活性随着乙醇浓度的增加而增加，并按以下顺序：乙醇，异丙醇和正丙醇。所有醇的活性随着试验条件的苛刻程度的增加而降低，即，在处理前培养时间较长，接种物水平较高。用于评估皮肤上细菌生长的接触印记和剥离方法清楚地表明，酒精处理减少但不能消除接种的病原体。研究发现，在某些环境条件下，皮肤上的病原体可能存在较长的寿命（数小时）。这一观察结果强烈表明，即使没有发生重复病原体污染的机会，频繁洗手也是必要的感染控制措施。
The feasibility of using pathogen-contaminated pig skin as a model substrate for evaluating skin disinfectants was demonstrated. A test methodology is described that is safe, convenient to use, and adaptable to a variety of hand-washing conditions. The treatment protocol, pathogen contamination conditions, and application technique variables can all be carefully controlled to simulate clinical use conditions, and application technique variables can all be carefully controlled to simulate clinical use conditions. The number of organisms transferred by contact was compared with the total organism count on the pig skin. The quantity of organisms transferred ranged from 10 to 60% of the total organisms, depending on the nature of the contamination conditions. The cumulative results of multiple imprint and stripping measurements were consistent with the concentration of inoculated organisms. Tests with alcohol solutions validated the methodology and clearly showed the dependence of topical antimicrobial activity on both the concentration and structure of the alcohol. Activity increased with increasing alcohol concentration and in the following order: ethanol, isopropanol, and n-propanol. All of the alcohols became less active as the severity of the test conditions was increased, i.e., higher inoculum levels for a longer incubation time before treatment. The contact imprint and stripping methods used to evaluate bacterial growth on the skin clearly showed that the alcohol treatments reduced but did not eliminate the inoculated pathogens. It was found that long lifetimes (several hours) for pathogens on the skin are possible under some environmental conditions. This observation strongly suggests that frequent hand washing is a necessary infection control practice even when opportunities for repeated pathogen contamination have not occurred.