Application of Brassica napus hairy root cultures for phenol removal from aqueous solutions
Application of Brassica napus hairy root cultures for phenol removal from aqueous solutions
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DOI：
10.1016/j.chemosphere.2008.04.003
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发表时间：
2008-07-01
期刊：
影响因子：
8.8
通讯作者：
Agostini, Elizabeth
中科院分区：
文献类型：
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作者：
Coniglio, Maria S.;Busto, Victor D.;Agostini, Elizabeth
酚类化合物存在于几个行业的排水中，是有害的污染物，对人类健康有潜在的危险。在这项工作中，我们研究了从水中去除苯酚使用甘蓝型油菜毛状根作为酶的来源，如过氧化物酶，这是能够氧化苯酚。这些毛状根进行了调查，他们的耐受性高毒性浓度的苯酚和参与他们的过氧化物酶同工酶在去除苯酚。根生长正常，在培养基中含有苯酚的浓度不超过100毫克升（-1），不添加过氧化氢。然而，根能够去除苯酚浓度高达500毫克l（-1），在H2 O2的存在下，达到高去除效率，在1小时内的处理和广泛的pH值（4-9）。毛状根可以重复使用，至少，三到四个连续的循环。过氧化物酶活性逐渐下降到约20%的控制，在第五个周期。碱性和近中性同功酶（BNP）沿着循环时间的延长而下降，酸性同功酶（AP）无变化。虽然这两组同工酶都参与了苯酚的去除，但AP对苯酚的亲和力和催化效率高于BNP。此外，苯酚处理后，AP保留更多的活性比BNP。因此，AP似乎是一个很有前途的同工酶，苯酚去除和连续处理的应用。此外，酶分离可能不是必需的，并且整个毛状根可能构成用于去污过程的较便宜的酶系统。(C)2008爱思唯尔有限公司保留所有权利。
Phenolic compounds present in the drainage from several industries are harmful pollutants and represent a potential danger to human health. In this work we have studied the removal of phenol from water using Brassica napus hairy roots as a source of enzymes, such as peroxidases, which were able to oxidise phenol. These hairy roots were investigated for their tolerance to highly toxic concentrations of phenol and for the involvement of their peroxidase isoenzymes in the removal of phenol. Roots grew normally in medium containing phenol in concentrations not exceeding 100 mg l(-1), without the addition of H2O2. However, roots were able to remove phenol concentrations up to 500 mg l(-1), in the presence of H2O2, reaching high removal efficiency, within 1 h of treatment and over a wide range of pH (4-9).Hairy roots could be re-used, at least, for three to four consecutive cycles. Peroxidase activity gradually decreased to approximately 20% of the control, at the fifth cycle. Basic and near neutral isoenzymes (BNP) decreased along time of recycling while acidic isoenzymes (AP) remained without changes. Although both group of isoenzymes would be involved in phenol removal, AP showed higher affinity and catalytic efficiency for phenol as substrate than BNP. In addition, AP retained more activity than BNP after phenol treatment. Thus, AP appears to be a promising isoenzyme for phenol removal and for application in continuous treatments. Furthermore, enzyme isolation might not be necessary and the entire hairy roots, might constitute less expensive enzymatic systems for decontamination processes. (C) 2008 Elsevier Ltd. All rights reserved.