Nasal provocation test (NPT) with isolated and associated dermatophagoides pteronyssinus (Dp) and endotoxin lipopolysaccharide (LPS) in children with allergic rhinitis (AR) and nonallergic controls.
Nasal provocation test (NPT) with isolated and associated dermatophagoides pteronyssinus (Dp) and endotoxin lipopolysaccharide (LPS) in children with allergic rhinitis (AR) and nonallergic controls.
复制标题
对过敏性鼻炎 (AR) 儿童和非过敏对照儿童进行鼻激发试验 (NPT)，使用分离的和相关的屋尘螨 (Dp) 和内毒素脂多糖 (LPS)。
DOI：
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复制
发表时间：
2004
影响因子：
7.2
通讯作者：
D. Solé
中科院分区：
文献类型：
--
作者：
C. Braga;M. Rizzo;C. Naspitz;D. Solé
背景
吸入内毒素可增强致敏哮喘患者吸入变应原后的炎性呼吸道反应(S)。
客观化
目的：评价儿童常年性变应性鼻炎(PAR)鼻腔内滴注屋尘螨(DP)、内毒素(LPS)及DP+LPS的疗效。
方法
10例PAR儿童(DP皮肤点刺试验阳性)和10例非过敏性对照(C)接受鼻腔激发试验(NPT)，采用主动前鼻测压和鼻腔总阻力(TNR)测量。首先用组胺(H；0.03~16.0 mg/mL)进行NPTS，然后至少每周间隔一次，用DP(1/100,000~1/2.5)、LPS(1~500 mg/mL)和DP+LPS进行NPTS。在NPT+DP+LPS过程中，DP浓度保持恒定(1/100,000；1/10,000；1/1000)，并与不同浓度的LPS(1，5，10，20 mg/mL)联合使用。当TNR达到基础TNR的两倍时，NPT被认为是正的。
结果
所有AR患儿的H和DP NPT均为阳性。C组H NPT阳性率为60%，DP NPT均为阴性。仅有30%的AR患儿NPT+内毒素阳性。当DP浓度为1/1000，内毒素浓度为5、10、20微克/毫升时，90%的AR患者NPT+DP+LPS呈阳性。在60%的AR患者中，观察到这种正相关与DP浓度低于NPT与DP之间的浓度。在NPT治疗后，所有儿童的肺功能测试均无变化。
结论
这项研究表明，脂多糖增强了过敏原刺激对鼻腔气流的影响。变应性鼻炎患者每天吸入过敏原和内毒素确实会增加鼻腔反应性。
BACKGROUND
Inhalation of endotoxin may enhance inflammatory airway response in sensitized asthmatics persons after allergen(s) inhalation.
OBJECTIVE
To evaluate nasal response to intranasal instillation of Dermatophagoides pteronyssinus (Dp), endotoxin (LPS), and to Dp+LPS in children with perennial allergic rhinitis (PAR).
METHODS
10 PAR children (positive skin prick test to Dp) and 10 nonallergic controls (C) undergoing nasal provocation test (NPT), who were quantified by active anterior rhinomanometry and measurement of Total Nasal Resistance (TNR). The NPTs were initially performed with histamine (H; 0.03 to 16.0 mg/mL), and then, at least at weekly intervals, the NPTs were done with Dp (1/100,000 to 1/2.5), LPS (1 to 500 mg/mL) and to Dp+LPS. During NPT with Dp+LPS, Dp concentration was kept constant (1/100,000; 1/10,000; 1/1,000) and was combined with different concentrations of LPS (1, 5, 10, 20 mg/mL). The NPT was considered positive when TNR reached twice the basal TNR.
RESULTS
H and Dp NPTs were positive in all AR children. In group C, H NPT was positive in 60% and Dp NPT was negative in all children. NPT with LPS was positive only in 30% of the AR children. NPT with Dp+LPS was positive in 90% of the AR patients in Dp concentration of 1/1,000 and in LPS concentrations of 5, 10, and 20 mcg/ mL. This positive association was observed with Dp concentrations lower than those obtained during NPT with Dp in 60% of AR patients. There were no changes in pulmonary function tests in all children after NPT.
CONCLUSIONS
This study suggests that LPS enhances the effects of allergen challenges on nasal airflow. The daily inhalation of allergens plus endotoxin in AR patients does increase the nasal responsiveness.
影响因子：
4.4
作者：
Peter S. Tobias;K. Soldau;L. Kline;Jae Lee;K. Kato;T. Martin;R. Ulevitch
通讯作者：
Peter S. Tobias;K. Soldau;L. Kline;Jae Lee;K. Kato;T. Martin;R. Ulevitch
DOI：
10.1152/ajplung.1996.270.5.l736
发表时间：
1996-05-01
影响因子：
4.9
作者：
Dubin, W;Martin, TR;Hasday, JD
通讯作者：
Hasday, JD
DOI：
10.1016/s0021-9258(18)81700-8
发表时间：
1989-06
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
P. Tobias;K. Soldau;R. Ulevitch
通讯作者：
P. Tobias;K. Soldau;R. Ulevitch