Biomaterial based treatment of osteoclastic/osteoblastic cell imbalance - Gelatin-modified calcium/strontium phosphates
Biomaterial based treatment of osteoclastic/osteoblastic cell imbalance - Gelatin-modified calcium/strontium phosphates
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DOI：
10.1016/j.msec.2019.109933
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发表时间：
2019-11-01
影响因子：
7.9
通讯作者：
Hanke, Thomas
中科院分区：
文献类型：
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作者：
Kruppke, Benjamin;Wagner, Alena-Svenja;Hanke, Thomas
骨质疏松骨代表其再生的困难条件，特别是在骨折的情况下。本研究针对骨质疏松骨的特殊需求，重点研究明胶改性磷酸钙、磷酸锶的可降解骨替代材料。从材料中释放的锶离子应该通过离子释放直接刺激成骨细胞，或者通过增加破骨细胞的存在和活性在材料吸收后间接刺激成骨细胞，从而刺激成骨细胞。一种新的多孔材料由磷酸钙、磷酸锶和在明胶存在下沉淀的磷酸钙/锶的混合相生产。最初，在标准含钙 (2.0 mM) 和低钙 (0.4 mM) 基本必需培养基中分析离子释放。在材料旁边培养人外周血单核细胞导致破骨细胞样细胞的形成，能够迁移、融合和分化。特别是，形态学和实时定量聚合酶链反应（RT-qPCR）证明，混合明胶修饰的磷酸钙/锶可以促进破骨细胞生成。正是这种材料使得在该材料上培养的人骨髓基质细胞具有最佳的成骨反应。对骨替代材料的研究表明，骨替代材料积极参与骨形态发生单元的细胞平衡。
Osteoporotic bone represents - particularly in case of fractures - difficult conditions for its regeneration. In the present study, the focus was put on a degradable bone substitute material of gelatin-modified calcium and strontium phosphates facing the special demands of osteoporotic bone. The release of strontium ions from the material ought to stimulate osteoblastogenesis either direct by ion release or indirect after material resorption by increased presence and activity of osteoclasts, which subsequently stimulate osteoblasts.A new porous material was produced from calcium phosphate, strontium phosphate and a mixed phase of calcium/strontium phosphate precipitated in presence of gelatin. Initially, ion release was analyzed in standard-calcium containing (2.0 mM) and low-calcium (0.4 mM) minimum essential medium. The cultivation of human peripheral blood mononuclear cells next to the material led to formation of osteoclast-like cells, able to migrate, fuse, and differentiate. Especially, the mixed gelatin-modified calcium/strontium phosphate allowed osteoclastogenesis as proven morphologically and by real-time quantitative polymerase chain reaction (RT-qPCR). It was precisely this material that led to the best osteoblastic reaction of human bone marrow stromal cells cultured on the material. The investigations of the bone substitute material indicate active involvement in the balance of cells of the bone morphogenetic unit.