Tetrahydrobiopterin-dependent nitrite oxidation to nitrate in isolated rat hepatocytes.
Tetrahydrobiopterin-dependent nitrite oxidation to nitrate in isolated rat hepatocytes.
复制标题
离体大鼠肝细胞中四氢生物蝶呤依赖性亚硝酸盐氧化为硝酸盐。
DOI：
10.1016/0014-5793(93)80644-a
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发表时间：
1993
期刊：
影响因子：
3.5
通讯作者：
LancasterJr,JR
中科院分区：
文献类型：
--
作者：
Kim,YM;LancasterJr,JR
We have found that isolated rat hepatocytes and cell extracts catalyze the stoichiometric conversion of nitrite (either as a product of the oxidation of endogenously synthesized nitric oxide or added as sodium nitrite) to nitrate, which in extracts requires the presence of tetrahydrobiopterin and is inhibited by cyanide but apparently not by carbon monoxide. The reaction is sensitive to heat denaturation and does not involve oxidation of NO2−by a peroxidative or radical oxygen mechanism. These results indicate the presence of a hitherto undescribed mammalian mechanism of inorganic nitrogen oxide oxidation that may be a protective mechanism against one potentially damaging effect of endogenous 'NO production (NO2−formation), and also that assays of NO formation based on NO2−determination alone may be an inaccurate measurement of this activity.
