PRR5 (PSEUDO-RESPONSE REGULATOR 5) plays antagonistic roles to CCA1 (CIRCADIAN CLOCK-ASSOCIATED-1) in Arabidopsis thaliana
PRR5 (PSEUDO-RESPONSE REGULATOR 5) plays antagonistic roles to CCA1 (CIRCADIAN CLOCK-ASSOCIATED-1) in Arabidopsis thaliana
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DOI：
10.1271/bbb.69.426
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发表时间：
2005-02-01
影响因子：
1.6
通讯作者：
Mizuno, T
中科院分区：
文献类型：
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作者：
Fujimori, T;Sato, E;Mizuno, T
一般来说，生物钟（或振荡器）是许多生物体昼夜节律的核心。在模式高等植物拟南芥中，最佳的时钟元件候选者是CCA 1（CIRCADIAN CLOCK-ASSOCIATED 1）和LHY（LATE ELLONGATED HYPOCOTYL），它们是同源的Myb相关转录因子。还认为TOC 1（CAB表达1的定时）是中央振荡器的另一个组成部分。在这方面，我们一直在表征一个小家族的蛋白质，命名为拟南芥假反应调节因子（PRR 1，PRR 3，PRR 5，PRR 7和PRR 9），基于这样的事实，即其中一个成员（PRR 1）与TOC 1相同。然而，目前尚不确定其他PRR家族成员是否也涉及时钟功能本身。为了解决这个问题，在这项研究中，我们研究了CCA 1时钟组件和PRR家族成员之一，PRR 5之间的功能相互作用，采用过表达的CCA 1和PRR 5基因的转基因株系。将提供PRR 5对推定的CCA 1时钟组分起拮抗作用的证据。
In general, the clock (or oscillator) is central to circadian rhythms in many organisms. In the model higher plant Arabidopsis thaliana, the best candidates forclock components are CCA1 (CIRCADIAN CLOCK-ASSOCIATED 1) and LHY (LATE ELONGATED HYPOCOTYL), which are homologous Myb-related transcription factors. It is also believed that TOC1 (TIMING OF CAB EXPRESSION 1) is another component of the central oscillator. In this connection, we have been characterizing a small family of proteins, designated ARABIDOPSIS PSEUDO-RESPONSE REGULATOR (PRR1, PRR3, PRR5, PRR7, and PRR9), based on the fact that one of the members (PRR1) is identical to TOC1. Nevertheless, it is not yet certain whether other PRR family members are also implicated in clock function per se. To address this issue, in this study we examined a functional interaction between the CCA1 clock component and one of the PRR family members, PRR5, by employing transgenic lines overexpressing both the CCA1 and PRR5 genes. Evidence will be provided that PRR5 plays an antagonistic role(s) to the putative CCA1 clock component.