RED CELL AGGREGATION BY MACRO MOLECULES ROLES OF SURFACE ADSORPTION AND ELECTROSTATIC REPULSION
RED CELL AGGREGATION BY MACRO MOLECULES ROLES OF SURFACE ADSORPTION AND ELECTROSTATIC REPULSION
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DOI：
10.1002/jss.400010418
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发表时间：
1973-01-01
期刊：
影响因子：
--
通讯作者：
JAN K-M
中科院分区：
文献类型：
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作者：
CHIEN S;JAN K-M
通过显微镜计数和光反射法定量测定不同分子大小和浓度的葡聚糖对正常和神经氨酸酶处理的人红细胞（RBC）的聚集。还研究了右旋糖酐溶液的离子强度和阳离子价态的变化对红细胞聚集的影响。红细胞聚集的数据与zeta电位的测量细胞电泳和细胞间的距离，在rouleaux的电子显微镜。利用胶体化学的经典方程和新发展的知识，由实验数据计算了相邻细胞表面（Fe）之间的静电斥力。引起RBC聚集的大分子桥连力（Fb）也被推导为葡聚糖浓度的函数。引起RBC解聚的其他力是机械剪切力（Fs）和RBC膜弯曲力（Fm）。RBC聚集的净力等于Fb-Fe- Fm- Fs.这种涉及表面力平衡的聚集模型可以解释影响细胞聚集的因素的已知实验结果。有人提出，这种细胞表面之间的力平衡可能适用于其他细胞或颗粒系统，它可能是在许多生理功能的根本重要性。
Aggregation of normal and neuraminidase‐treated human red blood cells (RBC) by dextrans of various molecular sizes and concentrations was quantified by microscopic counting and light reflectometry. The influences of variations in the ionic strength and the cationic valency of the dextran solution on RBC aggregation were also investigated. The data on RBC aggregation were correlated with measurements of the zeta potential by cell electrophoresis and the intercellular distance in the rouleaux by electron microscopy. With the use of the classical equations and newly developed knowledge in colloid chemistry, the electrostatic repulsive force between adjacent cell surfaces (Fe) was calculated from the experimental data. The macromolecular bridging force causing RBC aggregation (Fb) has also been derived as a function of dextran concentration. Other forces that cause RBC disaggregation are the mechanical shearing force (Fs) and the RBC membrane bending force (Fm). The net force for RBC aggregation is equal to Fb– Fe– Fm– Fs. This model of aggregation involving force balance at the surface can explain known experimental results on factors influencing cell aggregation. It is proposed that such force balance between cell surfaces may be applicable in other cell or particulate systems and that it may be of fundamental importance in many physiological functions.