Restricted tissue tropism and acquired resistance to Sindbis viral vector expression in the absence of innate and adaptive immunity.
Restricted tissue tropism and acquired resistance to Sindbis viral vector expression in the absence of innate and adaptive immunity.
复制标题
在缺乏先天性和适应性免疫的情况下，组织向性受到限制并获得了对辛德比斯病毒载体表达的抗性。
DOI：
10.1038/sj.gt.3302973
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发表时间：
2007
期刊：
影响因子：
5.1
通讯作者：
Meruelo,D
中科院分区：
文献类型：
--
作者：
Tseng,J-C;Zheng,Y;Yee,H;Levy,DE;Meruelo,D
Our previous studies suggest that replication-defective Sindbis vectors might be promising agents for specific tumor targeting and detection. However, the effects of innate and/or adaptive anti-viral immunity, in particular, the IFN-I/STAT1 signaling pathway, may impact their therapeutic potential. Using a bioluminescent imaging system, we demonstrate that although most normal cells are not permissively transduced by replication-defective Sindbis vector, transduction of liver non-sinusoidal endothelial occurs the first time IFN-I/STAT1 signaling deficient mice are inoculated with the vector. Transduction of some cells is not surprising since STAT1 knockout animals show significant delay in IFN responses such as the production of IFN-α/β and transcriptional activation of several anti-viral genes (IRF7, RIG-I, PKR, TLR3, USP18, ISG15). However, beyond the initial vector transduction, which resolves rapidly, secondary inoculums of Sindbis vectors do not transduce any liver cells, suggesting that an alternative antiviral pathway may protect against further transduction. Other known signaling pathways were examined using mice lacking functional TLR3, tumor necrosis factorαR or nuclear factor-kappa B (p50). Surprisingly, none of those pathways seem to play a significant role in anti-Sindbis responses. Thus it appears that in vivo, in contrast to the ready transduction of tumor cells, transduction of normal cells by replication-defective Sindbis vector is limited, possibly by a novel mechanism.
