CELL SORTING AND CHONDROGENIC AGGREGATE FORMATION IN MICROMASS CULTURE
CELL SORTING AND CHONDROGENIC AGGREGATE FORMATION IN MICROMASS CULTURE
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DOI：
10.1016/0012-1606(87)90314-9
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发表时间：
1987-08-01
影响因子：
2.7
通讯作者：
WOLPERT, L
中科院分区：
文献类型：
--
作者：
COTTRILL, CP;ARCHER, CW;WOLPERT, L
在发育中的肢体中软骨分化的一个基本特征是形成前软骨细胞凝聚。在肢芽间充质的微团培养物中，发生了明显相似的软骨形成前细胞聚集过程，形成细胞聚集体，通常分化成软骨结节。我们研究了微团培养中聚集体形成的过程，使用潜在的软骨形成和非软骨形成细胞类型的嵌合体混合物。研究了两个系统：远端和近端肢体间充质细胞的混合物和远端肢体细胞与禽腱成纤维细胞的混合物。在这两种情况下，已经制备了每种细胞类型的不同比例的培养物。结果表明，体外聚集体的形成是细胞分选过程的结果，该过程可能涉及发育肢体内广泛不同空间来源的前软骨细胞。这与体内软骨形成前冷凝形成对比，其中没有细胞分选的证据（Searls，R. L.（1967），J. Exp. Zool.166，39-50）。然而，我们的研究结果表明，细胞表面的差异发生在明显未分化的肢体间充质。结果还表明，间充质细胞聚集体必须达到一个阈值大小之前，软骨形成可以进行。此外，结果表明，在某些培养条件下，非软骨细胞将形成聚集体。
A fundamental feature of cartilage differentiation in the developing limb is the formation of a prechondrogenic cell condensation. An apparently similar process of prechondrogenic cell aggregation occurs in micromass cultures of limb bud mesenchyme with the formation of cellular aggregates which often differentiate into cartilage nodules. We have investigated the process of aggregate formation in micromass culture using chimaeric mixtures of potentially chondrogenic and nonchondrogenic cell types. Two systems were studied: mixtures of distal and proximal limb mesenchyme cells and mixtures of distal limb cells with avian tendon fibroblasts. In both cases cultures of varying proportions of each cell type have been prepared. The results demonstrate that aggregate formation in vitro is the consequence of a cell sorting process which can involve prechondrogenic cells of widely different spatial origins within the developing limb. This contrasts with in vivo prechondrogenic condensation in which there is no evidence of cell sorting (Searls, R. L. (1967), J. Exp. Zool. 166, 39-50). However, our findings do indicate that cell surface differences occur in apparently undifferentiated limb mesenchyme. The results also suggest that mesenchymal cell aggregates must achieve a threshold size before chondrogenesis can proceed. In addition, the results show that under some culture conditions nonchondrogenic cells will form aggregates.