A novel rat model of brachial plexus injury with nerve root stumps
A novel rat model of brachial plexus injury with nerve root stumps
复制标题
一种新型大鼠臂丛神经根残端损伤模型
DOI：
10.1016/j.jneumeth.2017.11.004
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发表时间：
2018-02-01
影响因子：
3
通讯作者：
Gu, Liqiang
中科院分区：
文献类型：
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作者：
Fang, Jintao;Yang, Jiantao;Gu, Liqiang
背景：臂丛神经损伤（brachial plexus injury， BPI）后，C5-C6神经根通常不会发生撕脱，因此可以作为神经移植的供体。到目前为止，还没有合适的动物模型来评估残存的神经根残端。因此，本研究的目的是建立并评价BPI大鼠神经根残端保留模型。新方法：断裂组在臂丛完全暴露后，用周围肌肉夹持C5-T1神经根近端，用力猛拉远端，与假手术组比较。为了验证该模型，测量C5-T1神经根残端长度，并对最短残端及相应脊髓进行组织学观察。结果：C5神经根残端最短。组织学检查显示神经纤维不规则性增强，连续性下降；髓鞘轴突的数量和直径以及髓鞘的厚度随着时间的推移而显著减少。运动神经元的存活减少，运动神经元的死亡可能与凋亡过程有关。与现有方法的比较：我们的模型可以成功地建立神经根残端牵引的BPI模型，可以模拟损伤机制。而其他模型涉及根撕脱或远端神经横断破裂。结论：该模型可用于神经根残端评价，并为今后通过神经根残端进行神经保护提供新的治疗策略。(C) 2017年Elsevier B.V.出版
Background: The C5-C6 nerve roots are usually spared from avulsion after brachial plexus injury (BPI) and thus can be used as donors for nerve grafting. To date, there are no appropriate animal models to evaluate spared nerve root stumps. Hence, the aim of this study was to establish and evaluate a rat model with spared nerve root stumps in BPI.New method: In rupture group, the proximal parts of C5-T1 nerve roots were held with the surrounding muscles and the distal parts were pulled by a sudden force after the brachial plexus was fully exposed, and the results were compared with those of sham group. To validate the model, the lengths of C5-T1 spared nerve root stumps were measured and the histologies of the shortest one and the corresponding spinal cord were evaluated.Results: C5 nerve root stump was found to be the shortest. Histology findings demonstrated that the nerve fibers became more irregular and the continuity decreased; numbers and diameters of myelinated axons and thickness of myelin sheaths significantly decreased over time. The survival of motoneurons was reduced, and the death of motoneurons may be related to the apoptotic process.Comparison with existing method(s): Our model could successfully create BPI model with nerve root stumps by traction, which could simulate injury mechanisms. While other models involve root avulsion or rupturing by distal nerve transection.Conclusions: This model would be suitable for evaluating nerve root stumps and testing new therapeutic strategies for neuroprotection through nerve root stumps in the future. (C) 2017 Published by Elsevier B.V.