Genetic analysis of opaque2 modifier loci in quality protein maize
Genetic analysis of opaque2 modifier loci in quality protein maize
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DOI：
10.1007/s00122-008-0762-y
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发表时间：
2008-07-01
影响因子：
5.4
通讯作者：
Larkins, Brian A.
中科院分区：
文献类型：
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作者：
Holding, David R.;Hunter, Brenda G.;Larkins, Brian A.
优质蛋白玉米(QPM)是通过选择遗传修饰剂将opaque2(O2)突变体的淀粉胚乳转化为坚硬的玻璃体表型而创造出来的。遗传分析表明，存在多种未连接的O2修饰物(OPM)，但它们的身份和作用方式尚不清楚。利用两个独立开发的QPM品系，我们将几个主要的OPM QTL定位到第1、7和9号染色体上。用从真正育种的O2子代获得的具有玻璃化和不透明核表型的RNA进行微阵列杂交，发现了一小群差异表达的基因，其中一些定位在OPM QTL上或附近。其中几个基因与乙烯和ABA信号相关，表明O2胚乳修饰与细胞程序性死亡之间存在潜在的联系。
Quality protein maize (QPM) was created by selecting genetic modifiers that convert the starchy endosperm of an opaque2 (o2) mutant to a hard, vitreous phenotype. Genetic analysis has shown that there are multiple, unlinked o2 modifiers (Opm), but their identity and mode of action are unknown. Using two independently developed QPM lines, we mapped several major Opm QTLs to chromosomes 1, 7 and 9. A microarray hybridization performed with RNA obtained from true breeding o2 progeny with vitreous and opaque kernel phenotypes identified a small group of differentially expressed genes, some of which map at or near the Opm QTLs. Several of the genes are associated with ethylene and ABA signaling and suggest a potential linkage of o2 endosperm modification with programmed cell death.