Hollow molecular imprinted polymers towards rapid, effective and selective extraction of caffeic acid from fruits
Hollow molecular imprinted polymers towards rapid, effective and selective extraction of caffeic acid from fruits
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中空分子印迹聚合物可快速、有效、选择性地从水果中提取咖啡酸
DOI：
10.1016/j.chroma.2016.10.006
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发表时间：
2016-10-28
影响因子：
4.1
通讯作者：
Chen, Xiaoqing
中科院分区：
文献类型：
--
作者：
Fan, Dengxin;Li, Hui;Chen, Xiaoqing
从复杂基质中快速、选择性地提取和富集痕量生物活性分析物对于高效、准确的定量分析具有重要意义。以咖啡酸（CA）为模板，4-乙烯基吡啶（4-VP）为功能单体，Fe3O4@SiO2为牺牲载体，制备了新型中空分子印迹聚合物（HMIPs）。采用傅里叶变换红外光谱（FT-IR）、透射电镜（TEM）、氮气吸附和热重分析（TGA）等手段对HMIPs的合成进行了验证。HMIPs的中空结构使其大比表面积（325.8m2/g）的识别位点集中在表面，与磁性分子印迹聚合物（MMIPs）和固体分子印迹聚合物相比，HMIPs的结合容量高（21.10mg/g），动力学结合速度快（35 min）。吸附平衡数据符合Freundlich方程，吸附过程符合准二级动力学模型。HIVIIPs的选择性性能良好。最后，HMIPs作为吸附剂，成功地从水果中快速，选择性地提取和富集CA，相对满意的回收率（85.6-103.5%）。结合高效液相色谱法测定猕猴桃、苹果、木瓜和杨梅4种水果中CA含量均小于1.0 μ g/g鲜果。结果表明，HMIP在从复杂基质中选择性萃取目标化合物方面具有优越性。(C)2016爱思唯尔B. V.保留所有权利。
Rapid and selective extraction and enrichment of trace bioactive analytes from complex matrices were of significant importance for efficient and accurate quantification. Here, novel hollow molecular imprinted polymers (HMIPs) were prepared using caffeic acid (CA) as template, 4-vinylpyridine (4-VP) as functional monomer, and Fe3O4@SiO2 as sacrificial support. Fourier transform infrared spectrometer (FT-IR), transmission electron microscopy (TEM), nitrogen adsorption and, thermo-gravimetric analysis (TGA) were used to verify the successful synthesis of HMIPs. Hollow structure with large surface area (325.8 m(2)/g) made most recognition sites locate on the surface of HMIPs, resulting in high binding capacity (21.10 mg/g) and fast kinetic binding (35 min) in comparison with magnetic MIPs (MMIPs) and solid MIPs. The equilibrium data fitted well to Freundlich equation and the adsorption process could be described by pseudo-second order model. The selectivity performance of HIVIIPs was favorable. Finally, HMIPs were successfully used as adsorbent to rapidly and selectively extract and enrich CA from fruits with a relatively satisfactory recovery (85.6-103.5%). Coupling with high-performance liquid chromatography (HPLC), the content of CA in four kinds of fruits (kiwifruit, apple, papaya and waxberry) was determined as less than 1.0 mu g/g fresh fruit. Results indicated the superiority of HMIPs in the selective extraction of target compound from complex matrices. (C) 2016 Elsevier B.V. All rights reserved.