Single-vesicle imaging reveals that synaptic vesicle exocytosis and endocytosis are coupled by a single stochastic mode
Single-vesicle imaging reveals that synaptic vesicle exocytosis and endocytosis are coupled by a single stochastic mode
复制标题
DOI：
10.1073/pnas.0707574105
复制
发表时间：
2007-12-18
影响因子：
11.1
通讯作者：
Ryan, T. A.
中科院分区：
文献类型：
--
作者：
Balaji, J.;Ryan, T. A.
35年来，神经末梢突触小泡再循环的本质一直是一个颇具争议的话题。在这里，我们报告了一种光学策略的使用，该策略允许在活的神经末梢中以单分子敏感性实时跟踪突触成分的吐出和取回。这种方法使我们能够研究突触小泡对单个动作电位的反应。我们的结果表明，在胞吐作用后，单个突触小泡的回收是一个延迟时间服从指数分布的随机过程，平均延迟时间约为14 S。我们认为，较慢过程的随机性质可能解释了快速内吞作用的证据，如支持接吻和跑的存在的证据。
The nature of synaptic vesicle recycling at nerve terminals has been a subject of considerable debate for >35 years. Here, we report the use of an optical strategy that allows the exocytosis and retrieval of synaptic components to be tracked in real time at single-molecule sensitivity in living nerve terminals. This approach has allowed us to examine the recycling of synaptic vesicles in response to single action potentials. Our results show that, after exocytosis, individual synaptic vesicles are retrieved by a stochastic process with an exponential distribution of delay times, with a mean time of approximate to 14 s. We propose that evidence for fast endocytosis, such as that proposed to support the presence of kiss-and-run, is likely explained by the stochastic nature of a slower process.