Relationship between periodontal pocket sulfide levels and subgingival species
Relationship between periodontal pocket sulfide levels and subgingival species
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DOI：
10.1034/j.1600-051x.2003.00377.x
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发表时间：
2003-11-01
影响因子：
6.7
通讯作者：
Socransky, SS
中科院分区：
文献类型：
--
作者：
Torresyap, G;Haffajee, AD;Socransky, SS
背景：与牙周病发病机制有关的许多物种都会产生挥发性硫化合物（VSC）。这项研究检查了同一牙周袋中硫化物水平和龈下细菌种类之间的关系。 材料和方法：对 20 名慢性牙周炎受试者在每颗牙齿的 6 个部位进行临床测量，以了解牙菌斑、牙龈炎、探诊出血、化脓、牙周袋深度和附着水平。使用棋盘 DNA-DNA 杂交技术，对取自每颗牙齿近中面的龈下菌斑样本单独分析 40 种细菌的含量。使用 Diamond Probe/Perio 2000 系统在同一地点测量硫化物水平。分别对每个受试者中硫化物阳性和阴性位点的临床和微生物学数据进行平均，然后在受试者之间进行平均。使用 Wilcoxon 符号秩检验寻找硫化物阳性和阴性位点之间临床和微生物参数的显着性差异。结果：硫化物阴性和阳性位点的平均总 DNA 探针计数 (x10(5), +/-SEM) 分别为 44.0+/-9.9 和 65.0+/-13.3 (p
Background: Many species implicated in the pathogenesis of periodontal disease produce volatile sulfur compounds (VSC). This investigation examined the relationship between levels of sulfide and subgingival bacterial species in the same periodontal pockets.Material and Methods: Twenty chronic periodontitis subjects were measured clinically at six sites per tooth for plaque, gingivitis, bleeding on probing, suppuration, pocket depth and attachment level. Subgingival plaque samples, taken from the mesial aspect of each tooth, were individually analyzed for their content of 40 bacterial species using checkerboard DNA-DNA hybridization. Sulfide levels were measured at the same sites using a Diamond Probe/Perio 2000 system. Clinical and microbiological data were averaged for sulfide-positive and -negative sites separately in each subject and then averaged across subjects. Significance differences in clinical and microbial parameters between sulfide-positive and -negative sites were sought using the Wilcoxon signed ranks test.Results: Mean total DNA probe counts (x10(5), +/-SEM) at sulfide-negative and -positive sites were 44.0+/-9.9 and 65.0+/-13.3, respectively (p