Structural features of the carboxy terminus of p60c-src that are required for the regulation of its intrinsic kinase activity.
Structural features of the carboxy terminus of p60c-src that are required for the regulation of its intrinsic kinase activity.
复制标题
p60c-src 羧基末端的结构特征是调节其内在激酶活性所必需的。
DOI：
10.20772/cancersci1985.78.12_1354
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发表时间：
1987
期刊：
影响因子：
--
通讯作者：
T. Takeya
中科院分区：
文献类型：
--
作者：
J. Kato;Y. Hirota;N. Nakamura;T. Takeya
p60 c-src和p60 v-src之间的主要结构差异在于羧基末端区域。我们在鸡p60 c-src基因的羧基端引入了取代、置换和缺失，并在鸡胚成纤维细胞中检测了其生化和生物学特性的影响。用p60 v-src的相应区域替换p60 c-src的氨基酸位置515后的羧基末端区域将p60 c-src转化为具有升高的激酶活性的转化蛋白。Tyr 527是p60 c-src的主要酪氨酸磷酸化位点，用苯丙氨酸取代导致激酶活性和转化能力增加。另一方面，515位后的缺失消除了激酶活性。这些结果揭示了p60 c-src的羧基端的结构特征，其内在的蛋白激酶活性的调节所需的。
The major structural difference between p60c-src and p60v-src resides in the carboxy-terminal region. We introduced substitution, replacement and deletion into the carboxy-terminal region of chicken p60c-src and examined the effects on its biochemical and biological properties in chicken embryo fibroblasts. The replacement of the carboxy-terminal region after amino acid position 515 of p60c-src with the corresponding region of p60v-src converted p60c-src to a transforming protein with elevated kinase activity. The substitution of Tyr527, which is a major tyrosine phosphorylation site of p60c-src, with phenylalanine resulted in an increase of kinase activity and in transforming ability. On the other hand, a deletion after position 515 abolished the kinase activity. These results revealed the structural features of the carboxy terminus of p60c-src that are required for the regulation of its intrinsic protein kinase activity.
影响因子：
56.9
作者：
COOPER, JA;GOULD, KL;HUNTER, T
通讯作者：
HUNTER, T
与多瘤病毒中型肿瘤抗原相关的 pp60c-src 分子氨基末端结构域内的酪氨酸磷酸化。
DOI：
10.1073/pnas.82.14.4568
发表时间：
1985
影响因子：
11.1
作者：
Yonemoto,W;Jarvis-Morar,M;Brugge,JS;Bolen,JB;Israel,MA
通讯作者：
Israel,MA
DOI：
10.1073/pnas.78.10.6013
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
SMART, JE;OPPERMANN, H;BISHOP, JM
通讯作者：
BISHOP, JM