Cyclin D3 in the mouse uterus is associated with the decidualization process during early pregnancy
Cyclin D3 in the mouse uterus is associated with the decidualization process during early pregnancy
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DOI：
10.1677/jme.0.0220091
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发表时间：
1999-02-01
影响因子：
3.5
通讯作者：
Dey, SK
中科院分区：
文献类型：
--
作者：
Das, SK;Lim, H;Dey, SK
在小鼠中，胚泡滋养外胚层和接受性子宫腔上皮之间的附着反应发生在妊娠第4天的2200-2300 h，随后基质细胞迅速转化为蜕膜细胞（蜕膜细胞反应）。这个过程也可以通过在假孕第4天子宫腔内注入油来实验性诱导（蜕膜瘤）。蜕膜细胞反应以细胞特异性方式与许多基因的上调和下调相关。使用mRNA差异显示，我们确定细胞周期蛋白D3的基因是在子宫中的囊胚附着反应过程中的位置上调。通过北方杂交测定，在第1-4天的早晨表达水平较低。原位杂交分析表明，在第1和第2天，信号主要定位于子宫上皮细胞，而信号检测到的基质和上皮细胞在第3和第4天。相反，随着第5、6和7天蜕膜化的开始和进展，子宫系膜和对系膜极的基质细胞中cyclin D3 mRNA的水平显著上调。然而，在第8天，信号主要定位于子宫系膜蜕膜床的基质细胞中。在这些天植入囊胚也表达细胞周期蛋白D3 mRNA。在炔雌醇处理的延迟着床小鼠中，在胚泡贴壁部位子宫细胞周期蛋白D3 mRNA水平适中，但随着雌二醇-17 β的着床开始而上调。然而，蜕膜细胞周期蛋白D3 mRNA的表达不依赖于囊胚的存在，因为在没有囊胚的情况下，在实验诱导的蜕膜瘤中也发生了表达增加。细胞周期蛋白D3在蜕膜化中的重要性在显示出缺陷的蜕膜化的Hoxa-10缺陷小鼠中进一步检查。Hoxa-10（-/-）子宫在第5天的细胞周期蛋白D3 mRNA的表达在假孕第4天应用促蜕膜刺激后严重受损。总的来说，结果表明，细胞周期蛋白D3可能是重要的过程中的蜕膜化。
In the mouse, the attachment reaction between the blastocyst trophectoderm and the receptive uterine luminal epithelium occurs at 2200-2300 h on day 4 of pregnancy and is rapidly followed by transformation of stromal cells into decidual cells (decidual cell reaction). This process can also be induced experimentally (deciduoma) by intraluminal oil infusion in the uterus on day 4 of pseudopregnancy. The decidual cell reaction is associated with up- and down-regulation of many genes in a cell-specific manner. Using mRNA differential display, we identified cyclin D3 as one of the genes that is upregulated in the uterus at the sites of blastocyst apposition during the attachment reaction. The levels of expression were low in the morning of days 1-4 as determined by Northern hybridization. In situ hybridization analysis showed that on days 1 and 2, signals were primarily localized in uterine epithelial cells, while signals were detected in both the stromal and epithelial cells on days 3 and 4. In contrast, with the initiation and progression of decidualization on days 5, 6 and 7, the levels of cyclin D3 mRNA were remarkably upregulated in stromal cells both at the mesometrial and the antimesometrial poles. However, on day 8, signals were primarily localized in stromal cells at the mesometrial decidual bed. Implanting blastocysts on these days also expressed cyclin D3 mRNA. In the progesterone-treated delayed implanting mice, the uterine levels of cyclin D3 mRNA were modest at the sites of blastocyst apposition, but were upregulated with the onset of implantation by estradiol-17 beta. However, the decidual expression of cyclin D3 mRNA nias not dependent on the presence of blastocysts, since increased expression also occurred in experimentally induced deciduoma in the absence of blastocysts. The importance of cyclin D3 in decidualization was further examined in Hoxa-10-deficient mice which show defective decidualization. The expression of cyclin D3 mRNA in Hoxa-10(-/-) uteri on day 5 was severely compromised after application of a deciduogenic stimulus on day 4 of pseudopregnancy. Collectively, the results suggest that cyclin D3 could be important for the process of decidualization.