Automated Dielectrophoretic Characterization of Mycobacterium smegmatis
Automated Dielectrophoretic Characterization of Mycobacterium smegmatis
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DOI：
10.1021/ac2002017
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发表时间：
2011-05-01
影响因子：
7.4
通讯作者：
Kirby, Brian J.
中科院分区：
文献类型：
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作者：
Hawkins, Benjamin G.;Huang, Chao;Kirby, Brian J.
我们通过自动 pDEP 细胞捕获实验报告了野生型和乙胺丁醇处理的耻垢分枝杆菌群体的阳性介电泳 (pDEP) 表征。通过测量羧酸盐改性聚苯乙烯微球和大肠杆菌来验证自动化技术。耻垢分枝杆菌的特征确定了一个关键的频率范围，其中乙胺丁醇的膜特异性作用导致细胞介电泳响应的变化。这项工作首次对分枝杆菌进行了此类表征，并强调了 DEP 测量在测量与化学处理或基因突变相关的分枝杆菌膜特性变化方面的潜力。
We report the positive dielectrophoretic (pDEP) characterization of wild-type and ethambutol-treated Mycobacterium smegmatis populations via automated pDEP cell trapping experiments. The automated technique was validated by measurements of carboxylate-modified polystyrene microspheres and Escherichia coli. The characterization of M. smegmatis identifies a key frequency regime where the membrane-specific action of ethambutol leads to a change in the cellular dielectrophoretic response. This work represents the first such characterization of Mycobacteria and highlights the potential for DEP measurements to measure changes in mycobacterial membrane properties associated with chemical treatments or genetic mutation.