Temporal and spatial immunolocalization of glucomannans in differentiating earlywood tracheid cell walls of Cryptomeria japonica
Temporal and spatial immunolocalization of glucomannans in differentiating earlywood tracheid cell walls of Cryptomeria japonica
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DOI：
10.1007/s00425-010-1189-7
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发表时间：
2010-07-01
期刊：
影响因子：
4.3
通讯作者：
Takabe, Keiji
中科院分区：
文献类型：
--
作者：
Kim, Jong Sik;Awano, Tatsuya;Takabe, Keiji
用免疫细胞化学方法研究了葡甘聚糖(GMS)在日本柳杉早期管胞分化中的沉积。在S(1)形成早期，GM在细胞壁的角部开始沉积，到S(1)形成时，在细胞壁上表现出不均匀分布。在S(2)形成的早期，S(2)层的GM标记有限，然后逐渐增加。在成熟的管胞中，S(1)和S(2)层之间的边界和细胞壁的最内侧部分显示出比细胞壁的其他部分更强的标记。与去乙酰化前相比，轻度碱处理的GM标记显著增加，GM在细胞壁中的分布更加均匀，这表明某些GM表位可能被乙酰化所掩盖。然而，去乙酰化后GM标记的变化直到S(2)形成的早期才非常明显，这表明在细胞壁形成的早期沉积在细胞壁中的GM含有的乙酰基可能比那些在细胞壁形成后期沉积的GM少。此外，在GM脱乙酰化前后，甚至在细胞壁形成完成后，两个样本的细胞壁中GM标记的密度都增加了。这一发现表明，作为管胞细胞老化过程的一部分，在细胞壁形成完成后，一些乙酰基可能会从转基因材料中去除。
We investigated the deposition of glucomannans (GMs) in differentiating earlywood tracheids of Cryptomeria japonica using immunocytochemical methods. GMs began to deposit at the corner of the cell wall at the early stages of S(1) formation and showed uneven distribution in the cell wall during S(1) formation. At the early stages of S(2) formation, limited GM labeling was observed in the S(2) layer, and then the labeling increased gradually. In mature tracheids, the boundary between the S(1) and S(2) layers and the innermost part of the cell wall showed stronger labeling than other parts of the cell wall. Deacetylation of GMs with mild alkali treatment led to a significant increase in GM labeling and a more uniform distribution of GMs in the cell wall than that observed before deacetylation, indicating that some GM epitopes may be masked by acetylation. However, the changes in GM labeling after deacetylation were not very pronounced until early stages of S(2) formation, indicating that GMs deposited in the cell wall at early stages of cell-wall formation may contain fewer acetyl groups than those deposited at later stages. Additionally, the density of GM labeling increased in the cell wall in both specimens before and after GM deacetylation, even after cell-wall formation was complete. This finding suggests that some acetyl groups may be removed from GMs after cell-wall formation is complete as part one of the tracheid cell aging processes.