A near-infrared fluorescent probe for fluorine ions and its application in the imaging of HepG2 cells
A near-infrared fluorescent probe for fluorine ions and its application in the imaging of HepG2 cells
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氟离子近红外荧光探针及其在HepG2细胞成像中的应用
DOI：
10.1007/s11434-011-4662-1
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发表时间：
2011-11-01
影响因子：
--
通讯作者：
Tang, Bo
中科院分区：
文献类型：
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作者：
Gong, Wei;Su, RuiXian;Tang, Bo
本研究利用荧光染料BODIPY和三氟甲苯之间的B-Se键，设计并合成了一种新的近红外荧光探针（BODIPY-Se）。该探针对F.检出限为7.4 × 10 ~（-8）mol/L。探针在NIR区域的激发和发射光谱避免了生物系统中存在的背景荧光干扰。HepG 2细胞的荧光成像结果表明，该探针可广泛应用于F.在活细胞中。
In this study, we have designed and synthesized a new near-infrared (NIR) fluorescent probe (BODIPY-Se) to detect fluorine ions (F-) using a B-Se bond to connect the fluorescent dye BODIPY and benzotrifluoride. The probe exhibited a highly selective fluorescence response to F. with a detection limit of 7.4 x 10(-8) mol/L. The excitation and emission spectra of the probe in the NIR region avoid background fluorescence interference present in biological systems. The fluorescent imaging of HepG2 cells demonstrated that the newly developed probe should be broadly applicable to detect F. in living cells.