Polymer degradation and in vitro release of a model protein from poly(D,L-lactide-co-glycolide) nano- and microparticles
Polymer degradation and in vitro release of a model protein from poly(D,L-lactide-co-glycolide) nano- and microparticles
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DOI：
10.1016/s0168-3659(03)00328-6
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发表时间：
2003-09-19
影响因子：
10.8
通讯作者：
Labhasetwar, V
中科院分区：
文献类型：
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作者：
Panyam, J;Dali, MA;Labhasetwar, V
本研究的目的是考察由聚(D，L-丙交酯-乙交酯)(50：50PLGA)制备的纳米和微米粒子的粒径对聚合物降解和蛋白质释放的影响。由于比表面积与体积比与颗粒大小成反比，因此推测颗粒的大小会影响聚合物的降解和包膜蛋白质的释放。以牛血清白蛋白为模型蛋白，采用多重包水型乳化溶剂挥发法制备了平均直径分别为0.1、1和10微米的PLGA纳米粒子和微球。这些粒子在37℃的磷酸盐缓冲盐水(pH 7.4,154 mm)中孵育，并在不同的时间点用扫描电子显微镜表征聚合物的相对分子质量、表面相关的聚乙烯醇含量(PVA)和粒子的表面拓扑结构。分析上述研究的上清液中释放的蛋白质和PVA含量。发现聚合物在纳米粒子和微米粒子中的降解都是两相的，最初的快速降解持续20-30天，然后是较慢的降解阶段。0.1um直径的纳米粒子对聚合物的降解率相对较高(P
The objective of the study was to investigate the effect of particle size of nano- and rnicroparticles formulated from poly(D,L-lactide-co-glycolide) (50:50 PLGA) on polymer degradation and protein release. Since the surface area to volume ratio is inversely proportional to the particle size, it is hypothesized that the particle size would influence the polymer degradation as well as the release of the encapsulated protein. PLGA nano- and microparticles of approximate mean diameters of 0.1, 1 and 10 mum, containing bovine serum albumin as a model protein, were formulated using a multiple water-in-oil-in-water emulsion solvent evaporation technique. These particles were incubated at 37 degreesC in phosphate-buffered saline (pH 7.4, 154 mM) and the particles were characterized at various time points for molecular weight of polymer, surface-associated polyvinyl alcohol content (PVA), and the particle surface topology using scanning electron microscopy. The supernatants from the above study were analyzed for the released protein and PVA content. Polymer degradation was found to be biphasic in both nano- and microparticles, with an initial rapid degradation for 20-30 days followed by a slower degradation phase. The 0.1 mum diameter nanoparticles demonstrated relatively higher polymer degradation rate (P