Transcription of hematopoietic-associated oncogenes in childhood leukemia.
Transcription of hematopoietic-associated oncogenes in childhood leukemia.
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发表时间：
1983-08
期刊：
影响因子：
11.2
通讯作者：
D. Rosson;A. Tereba
中科院分区：
文献类型：
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作者：
D. Rosson;A. Tereba
我们检测了几种逆转录病毒相关癌基因myc、rel、rasH、myb、src和erb在未培养的儿童白血病和正常造血细胞中的细胞同源物的转录表达，作为确定其正常功能和与人类肿瘤可能关联的第一步。在所有30个造血组织样本中检测到细胞myc特异性RNA，包括18个淋巴和髓系白血病，3个淋巴瘤，5个正常白细胞和4个细胞系。虽然表达水平在25倍范围内变化，但没有明显的基于细胞类型或疾病状态的一般模式。此外，在所有检测的细胞类型中，观察到一种单分子量的myc特异性RNA物种。rel和rasH基因的转录表达显示出类似的缺乏特异性，在所有检测的细胞类型中，rasH基因的表达水平都很低。在大多数样本中，Myb的表达是微弱的，尽管髓性白血病细胞的表达水平大约高出4倍。src在大多数样本中的表达相对较低，在少数不同的白血病样本中表达水平明显升高。Erb的表达在除两个急性骨髓性白血病样本外的所有样本中均未检测到。对一名治疗前myc、erb和src表达水平较高的患者的分析显示，在患者缓解期，erb和src表达显著降低，但myc表达没有显著降低。这些结果表明，原代人白血病细胞和正常白细胞一样，确实表达几种逆转录病毒相关癌基因的细胞同源物，一些白血病细胞高水平表达几种癌基因，其中一些基因在特定细胞亚群中表达差异。
We have examined the transcriptional expression of the cellular homologues of several retrovirus-associated oncogenes, myc, rel, rasH, myb, src, and erb, in uncultured childhood leukemia and normal hematopoietic cells, as a first step in determining their normal function and possible association with human neoplasia. Cellular myc-specific RNA was detected in all 30 samples of hematopoietic tissue examined, including 18 leukemias of both the lymphoid and myeloid series, three lymphomas, five normal leukocytes, and four cell lines. Although the level of expression varied over a 25-fold range, no general pattern based on cell type or disease state was evident. In addition, in all cell types examined, a single-molecular-weight myc-specific RNA species was observed. Transcriptional expression of the rel and rasH genes showed a similar lack of specificity, with the rasH gene being expressed at a low uniform level in all cell types examined. myb expression was marginally detectable in most samples, although the myeloid leukemia cells possessed approximately 4-fold higher levels. The expression of src was relatively low in most samples, with markedly elevated levels in a few diverse leukemia samples. erb expression was undetectable in all but two acute myelogenous leukemia samples. Analysis of one patient who had high levels of myc, erb, and src expression before therapy revealed a dramatic reduction in erb and src expression but not myc expression while the patient was in remission. These results indicate that primary human leukemia cells, as well as normal leukocytes, do express the cell homologues to several retrovirus-associated oncogenes, that some leukemia cells express high levels of several oncogenes, and that some of these genes are differentially expressed in specific subpopulations of cells.