Increased macrophage chemoattractant protein-1 in cerebrospinal fluid precedes and predicts simian immunodeficiency virus encephalitis.
Increased macrophage chemoattractant protein-1 in cerebrospinal fluid precedes and predicts simian immunodeficiency virus encephalitis.
复制标题
脑脊液中巨噬细胞趋化蛋白-1 的增加先于并预测猿猴免疫缺陷病毒脑炎。
DOI：
10.1086/323478
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发表时间：
2001
期刊：
影响因子：
--
通讯作者：
Clements,JE
中科院分区：
文献类型：
--
作者：
Zink,MC;Coleman,GD;Mankowski,JL;Adams,RJ;Tarwater,PM;Fox,K;Clements,JE
巨噬细胞趋化蛋白-1 (MCP-1) 可能是人类免疫缺陷病毒 (HIV) 脑炎中巨噬细胞流入大脑的关键触发因素。在这项研究中，感染猿猴免疫缺陷病毒并出现中度至重度脑炎的猕猴，早在接种后 28 天（即出现脑部病变之前），其脑脊液 (CSF) 中的 MCP-1 水平就显着高于血浆中的水平。相比之下，在未出现脑炎或未出现脑炎的猕猴中，CSF：血浆 MCP-1 比率保持在接种前水平。在大脑中的巨噬细胞和星形胶质细胞中检测到丰富的 MCP-1 蛋白和 mRNA。脑脊液中 MCP-1 增加的猕猴的巨噬细胞和小胶质细胞活化和浸润（CD68；P=.003）和星形胶质细胞活化（胶质纤维酸性蛋白；白质和灰质中分别为 P=.019 和 P=.031）标记物的表达显着增加。结果表明，CSF：血浆 MCP-1 比率可能是 HIV 引起的中枢神经系统疾病发展的一个有价值的预后标志物
Macrophage chemoattractant protein–1 (MCP-1) may be a key trigger for the influx of macrophages into the brain in human immunodeficiency virus (HIV) encephalitis. In this study, simian immunodeficiency virus–infected macaques that developed moderate-to-severe encephalitis had significantly higher MCP-1 levels in cerebrospinal fluid (CSF) than in plasma as early as 28 days after inoculation, which was before the development of brain lesions. In contrast, CSF:plasma MCP-1 ratios remained constant at preinoculation levels in macaques that developed minimal or no encephalitis. Abundant MCP-1 protein and mRNA were detected in both macrophages and astrocytes in the brain. Macaques with increased MCP-1 in CSF had significantly greater expression of markers of macrophage and microglia activation and infiltration (CD68; P=.003) and astrocyte activation (glial fibrillary acidic protein; P=.019 and P=.031 in white and gray matter, respectively). The results suggest that the CSF:plasma MCP-1 ratio may be a valuable prognostic marker for the development of HIV-induced central nervous system disease