Gene transfer into hypothalamic organotypic cultures using an adeno-associated virus vector
Gene transfer into hypothalamic organotypic cultures using an adeno-associated virus vector
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DOI：
10.1006/exnr.1999.7236
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发表时间：
1999-12-01
影响因子：
5.3
通讯作者：
Gainer, H
中科院分区：
文献类型：
--
作者：
Keir, SD;House, SB;Gainer, H
大鼠下丘脑切片培养物的器官型培养物成功地使用腺相关病毒载体转导。使用核靶向的Lac-Z作为报告基因，发现转导是非常有效的，发生在高达89%的特定细胞类型，催产素神经元，存在于培养的外植体。这些转导水平在转导后7天在培养的细胞中没有伴随任何有害作用。这种体外方法对于中枢神经系统中神经元中细胞特异性基因表达的研究应该是有价值的，对于中枢神经系统，没有同源（替代）细胞系，（C）1999 Academic Press。
Organotypic cultures of rat hypothalamic slice cultures were successfully transduced using adenoassociated viral vectors. Using nuclear-targeted Lac-Z as the reporter gene, transduction was found to be very effective, occurring in as high as 89% of a specific cell type, the oxytocin neurons, present in the cultured explants. These transduction levels were not accompanied by any deleterious effects in the cultured cells 7 days after transduction. Such an in vitro approach should be valuable for the study of cell-specific gene expression in neurons in the central nervous system for which there are no homologous (surrogate) cell lines, (C) 1999 Academic Press.