Long-range carbon-carbon connectivity via unsymmetrical indirect covariance processing of HSQC and HMBC NMR data
Long-range carbon-carbon connectivity via unsymmetrical indirect covariance processing of HSQC and HMBC NMR data
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DOI：
10.1002/mrc.1766
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发表时间：
2006-02-01
影响因子：
2
通讯作者：
Martin, GE
中科院分区：
文献类型：
--
作者：
Blinov, KA;Larin, NI;Martin, GE
最近证明，IDR-（反向直接响应）HSQC-TOCSY 数据集可以分解为负相位直接响应谱和正相位中继响应谱，然后可以对其进行不对称间接协方差处理，以消除由于分子质子 NMR 谱中的响应重叠而导致的伪影。使用实验上离散的 HSQC 和 HMBC 数据集，结果表明，一对 NMR 谱的不对称间接协方差处理提供了包含长程碳-碳连通性信息的表示。使用马钱子碱作为模型化合物演示了该方法。正如之前报道的那样，尽管由于质子响应重叠可能会出现伪影，但所得数据基本上没有伪影。版权所有 (c) 2005 John Wiley & Sons, Ltd.
It was recently demonstrated that an IDR- (Inverted Direct Response) HSQC-TOCSY data set could be decomposed into a negatively phased direct response spectrum and a positively phased relayed response spectrum that could then be subjected to unsymmetrical indirect covariance processing for the removal of artifacts due to response overlap in the proton NMR spectrum of the molecule. Using experimentally discrete HSQC and HMBC data sets, it is shown that unsymmetrical indirect covariance processing of the pairof NMR spectra affords a presentation containing long-range carbon-carbon connectivity information. The method is demonstrated using strychnine as a model compound. The resulting data are largely free of artifacts although artifacts can arise due to proton response overlap, as previously reported. Copyright (c) 2005 John Wiley & Sons, Ltd.