Molecular characterization and expression of CD2BP2 in Nile tilapia (Oreochromis niloticus) in response to Streptococcus agalactiae stimulus
Molecular characterization and expression of CD2BP2 in Nile tilapia (Oreochromis niloticus) in response to Streptococcus agalactiae stimulus
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尼罗罗非鱼 (Oreochromis niloticus) 响应无乳链球菌刺激的 CD2BP2 分子特征和表达
DOI：
10.1016/j.gene.2014.07.032
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发表时间：
2014-09-10
期刊：
影响因子：
3.5
通讯作者：
Wu，Zaohe
中科院分区：
文献类型：
--
作者：
Gan，Zhen;Wang，Bei;Wu，Zaohe
CD2BP2 （CD2胞浆尾结合蛋白2）是与CD2胞浆尾相互作用的蛋白之一，在CD2触发的T细胞活化和核剪接中起着至关重要的作用。关于CD2BP2的研究往往局限于少数哺乳动物，迄今为止关于鱼类CD2BP2的信息很少。从尼罗罗非鱼（Oreochromis niloticus）中克隆了一个CD2BP2基因（On-CD2BP2）。序列分析表明，On-CD2BP2 cDNA全长1429 bp，其中5'untranslated region (UTR) 111 bp, 3 ' untranslated region (UTR) 193 bp，开放阅读框1125 bp，编码374个氨基酸。在推导出的On-CD2BP2氨基酸序列中检测到GYF结构域和共识基序GPFXXXXMXXWXXXGYF两个重要的结构特征，推导出的On-CD2BP2基因组结构与已知的CD2BP2相似。采用荧光实时定量PCR技术分析了On-CD2BP2 mRNA在尼罗罗非鱼各组织中的表达情况。在健康尼罗罗非鱼中，On-CD2BP2转录本主要在头部肾脏和脾脏中检测到。接种灭活无乳链球菌疫苗后，免疫48 h后头部肾脏、脾脏和脑组织On-CD2BP2 mRNA表达显著上调。此外，免疫后On-CD2BP2具有明显的时间依赖性表达模式，免疫后24 h在脑、48 h在头肾和脾的表达达到最高水平。这是首次证实鱼类中存在CD2BP2同源基因，并研究其在细菌刺激下的组织分布和表达谱。这些发现表明On-CD2BP2可能在尼罗罗非鱼对细菌的免疫应答中发挥重要作用。
CD2BP2 (CD2 cytoplasmic tail binding protein 2), one of several proteins interacting with the cytoplasmic tail of CD2, plays a crucial role in CD2-triggered T cell activation and nuclear splicing. The studies on CD2BP2 have tended to be confined to a few mammals, and little information is available to date regarding fish CD2BP2. In this paper, a CD2BP2 gene (On-CD2BP2) was cloned from Nile tilapia,Oreochromis niloticus. Sequence analysis showed that the full length of On-CD2BP2 cDNA was 1429 bp, containing a 5′untranslated region (UTR) of 111 bp, a 3′-UTR of 193 bp and an open reading frame of 1125 bp which is encoding 374 amino acids. Two important structural features, a GYF domain and a consensus motif GPFXXXXMXXWXXXGYF were detected in the deduced amino acid sequence of On-CD2BP2, and the deduced genomic structure of On-CD2BP2 was similar to the known CD2BP2. The mRNA expression of On-CD2BP2 in various tissues of Nile tilapia was analyzed by fluorescent quantitative real-time PCR. In healthy Nile tilapia, the On-CD2BP2 transcripts were mainly detected in the head kidney and spleen. While vaccinated with inactivatedStreptococcus agalactiae, the On-CD2BP2 mRNA expression was significantly up-regulated in the head kidney, spleen and brain 48 h post immunization. Moreover, there was a clear time-dependent expression pattern of On-CD2BP2 after immunization and the expression reached the highest level at 24 h in the brain and 48 h in the head kidney and spleen. This is the first report of proving the presence of a CD2BP2 ortholog in fish, and investigating its tissue distribution and expression profile in response to bacterial stimulus. These findings indicated that On-CD2BP2 may play an important role in the immune response to bacteria in Nile tilapia.