AMPLIFICATION OF A CHIMERIC BACILLUS GENE IN CHLOROPLASTS LEADS TO AN EXTRAORDINARY LEVEL OF AN INSECTICIDAL PROTEIN IN TOBACCO
AMPLIFICATION OF A CHIMERIC BACILLUS GENE IN CHLOROPLASTS LEADS TO AN EXTRAORDINARY LEVEL OF AN INSECTICIDAL PROTEIN IN TOBACCO
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DOI：
10.1038/nbt0495-362
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发表时间：
1995-04-01
期刊：
影响因子：
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通讯作者：
MALIGA, P
中科院分区：
文献类型：
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作者：
MCBRIDE, KE;SVAB, Z;MALIGA, P
苏云金芽孢杆菌（Bacillus thuringiensis，Bt）晶体毒素是一种安全的生物杀虫剂，但其持效期短，对取食于植物组织内的害虫效果不佳。在植物中生产有效水平的这些蛋白质需要重新合成编码它们的基因。我们报道，在叶绿体中扩增未修饰的cry 1A（c）编码序列，每个细胞高达10，000个拷贝，导致烟草叶片中前所未有的3-5%的可溶性蛋白质作为原毒素积累。该植物对烟实夜蛾、谷实夜蛾和甜菜夜蛾幼虫有极强的毒力。由于质体转基因不通过花粉传播，这一报告对遏制Bt基因在作物植物中的影响。此外，高水平的杀虫蛋白的合成将有助于提高Bt抗性昆虫种群的管理水平。
Bacillus thuringiensis (Bt) crystal toxins are safe biological insecticides, but have short persistance and are poorly effective against pests that feed inside plant tissues. Production of effective levels of these proteins in plants has required resynthesis of the genes encoding them. We report that amplification of an unmodified crylA(c) coding sequence in chloroplasts up to similar to 10,000 copies per cell resulted in the accumulation of an unprecedented 3-5% of the soluble protein in tobacco leaves as protoxin. The plants were extremely toxic to larvae of Heliothis virescens, Helicoverpa zea, and Spodoptera exigua. Since the plastid transgenes are not transmitted by pollen, this report has implications for containment of Bt genes in crop plants. Furthermore, accummulation of insecticidal protein at a high level will facilitate improvement in the management of Bt resistant insect populations.