Determination of thermodynamic parameters of cadmium(II) association to glutathione using the fluorescent reagent FluoZin-1.
Determination of thermodynamic parameters of cadmium(II) association to glutathione using the fluorescent reagent FluoZin-1.
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DOI：
10.1016/j.ab.2010.03.030
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发表时间：
2010-07
影响因子：
2.9
通讯作者：
Shinya Ogawa;E. Yoshimura
中科院分区：
文献类型：
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作者：
Shinya Ogawa;E. Yoshimura
开发了一种基于试剂 FluoZin-1 测定镉 (II) [Cd(II)] 配合物条件缔合常数的方法，该试剂与 Cd(II) 形成荧光配合物。用谷胱甘肽滴定含有 Cd(II) 和 FluoZin-1 的溶液，同时测定 FluoZin-1 的荧光强度，以估计游离 Cd(II) 的水平。使用 Microsoft Excel 的 Solver 算法通过非线性最小二乘法对结果进行分析，得出 1:1 和 1:2 Cd(II)-谷胱甘肽复合物的条件关联常数。获得的值与先前使用等温滴定量热法报道的值一致。
A method was developed to determine the conditional association constants of cadmium(II) [Cd(II)] complexes based on the reagent FluoZin-1, which forms a fluorescent complex with Cd(II). A solution containing Cd(II) and FluoZin-1 was titrated with glutathione while determining fluorescence intensity of FluoZin-1 to estimate levels of free Cd(II). The results were analyzed with a nonlinear least-squares method using the Solver algorithm of Microsoft Excel to yield conditional association constants for 1:1 and 1:2 Cd(II)–glutathione complexes. The values obtained were consistent with those reported previously using isothermal titration calorimetry.