PIF MUTATION BLOCKS RECOMBINATION BETWEEN MITOCHONDRIAL RHO+ AND RHO- GENOMES HAVING TANDEMLY ARRAYED REPEAT UNITS IN SACCHAROMYCES-CEREVISIAE
PIF MUTATION BLOCKS RECOMBINATION BETWEEN MITOCHONDRIAL RHO+ AND RHO- GENOMES HAVING TANDEMLY ARRAYED REPEAT UNITS IN SACCHAROMYCES-CEREVISIAE
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DOI：
10.1073/pnas.80.17.5345
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发表时间：
1983-01-01
期刊：
影响因子：
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通讯作者：
KOLODYNSKI, J
中科院分区：
文献类型：
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作者：
FOURY, F;KOLODYNSKI, J
三个影响线粒体DNA重组的等位核突变体在S.酿酒酵母并分配到PIF基因座。在突变体中，通过连锁或非连锁等位基因之间的重组频率测量的一般重组是正常的。然而，pif突变阻止了整合到ρ +这些ρ-的标记（oli 1、oli 2、diu 1、ery、oxi 1、oxi 2）的基因组具有串联重复单元的基因组。因此，这些.rh. -基因组表征PIF依赖性重组系统。pif突变还揭示了存在由那些ρ-突变体使用的PIF非依赖性重组系统。重复单位反向排列的基因组。这种回文的标记-基因组表现出高整合频率进入ρ +基因组甚至在PIF突变的存在下。此外，PIF突变极大地增加了PIF ρ +菌株和PIF依赖性以及PIF非依赖性克隆ρ +之间的重组和ρ-基因组涉及至少2个不同的系统，其依赖于ρ的组织。基因组
Three allelic nuclear mutants affected in the recombination of mtDNA characterized in S. cerevisiae and assigned to the PIF locus. In the mutants, the general recombination measured by the recombination frequency between linked or unlinked alleles is normal. However, the pif mutations prevent the integration into the .rho.+ genome of the markers (oli1, oli2, diu1, ery, oxi1, oxi2) of those .rho.- genomes that have tandemly arrayed repeat units. Therefore, these .rho.- genomes characterize a PIF-dependent recombination system. The pif mutations also revealed the existence of a PIF-independent recombination system used by those .rho.- genomes that have an inverted organization of their repeat units. The markers of such palindromic .rho.- genomes exhibit high integration frequency into the .rho.+ genome even in the presence of the pif mutation. In addition, the pif mutations greatly increase suppressiveness in crosses between pif .rho.+ strains and PIF-dependent as well as PIF-independent .rho.- clones. The recombination between .rho.+ and .rho.- genomes involves at least 2 distinct systems that depend on the organization of the .rho.- genome.