Improving production of aromatic compounds in Escherichia coli by metabolic engineering
Improving production of aromatic compounds in Escherichia coli by metabolic engineering
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DOI：
10.1016/0167-7799(96)10033-0
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发表时间：
1996-07-01
影响因子：
17.3
通讯作者：
Berry, A
中科院分区：
文献类型：
--
作者：
Berry, A
原料转化为产品的效率是控制大规模发酵过程生产代谢物的商业可行性的主要因素。开发代谢产物过量生产的微生物菌株的传统方法依赖于仅放大所需产品的特定生物合成途径中的酶步骤。虽然这种方法在达到高产品浓度和产量方面通常是成功的，但进一步的改进(从而降低制造成本)只能通过将碳流从中央新陈代谢重新引导到产品形成途径来实现。这种对中央代谢途径的操纵有望产生新一代代谢物产生微生物。
The efficiency of conversion of raw material to product is a major factor controlling the commercial viability of large-scale fermentation processes for the production of metabolites. Traditional approaches to developing microbial strains for metabolite overproduction have relied on amplifying only the enzymatic steps within the specific biosynthetic pathway of a desired product. While this approach has generally been successful for reaching high product concentrations and yields, further improvements (and hence reduced manufacturing cost) can only be achieved by redirecting carbon flux from central metabolism to the product-forming pathway. Such manipulation of central metabolic pathways promises to deliver a new generation of metabolite-producing microorganisms.