Interaction of nuclear ERs and GPER in vitellogenesis in zebrafish
Interaction of nuclear ERs and GPER in vitellogenesis in zebrafish
复制标题
斑马鱼卵黄发生中核 ER 和 GPER 的相互作用
DOI：
10.1016/j.jsbmb.2019.01.013
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发表时间：
2019-05-01
影响因子：
4.1
通讯作者：
Lin, Haoran
中科院分区：
文献类型：
--
作者：
Chen, Yu;Tang, Haipei;Lin, Haoran
Estrogens exert their biological functions through the estrogen receptors (ERs). In zebrafish, three nuclear estrogen receptors (nERs) named ER alpha, ER beta 1 and ER beta 2 and one membrane-bound G protein-coupled estrogen receptor (GPER) are identified. Vitellogenin (Vtg) is predominantly expressed in liver and strongly response to the stimulation of estrogen. It has been proposed that all three nERs are functionally involved in vitellogenesis and ERa may act as the major mediator in teleost. However, the role of GPER and its interaction with nERs in this process are not yet defined in teleost species. In the present study, we provide genetic evidence for the functional significance of ERa that the expression of Vtg genes (vtg1, vtg2, vtg3) and their response to estradiol stimulation were significantly decreased in esr1 mutant zebrafish. Activation of ER beta 1 and ER beta 2 induced Vtg expression through ER alpha. Moreover, the involvement of GPER in vitellogenesis and its interaction with nERs in zebrafish were firstly proposed in this work. Activation of GPER induced Vtg genes expression while inhibition of GPER significantly attenuated the estrogenic effect on Vtg. Both treatments altered the expression levels of nERs, suggesting GPER acts interactively with nERs. Collectively, the involvement of both nERs and GPER in regulation of vitellogenesis is demonstrated. ER alpha is the central factor, acting interactively with ER beta 1, ER beta 2 and GPER, and GPER regulates vitellogenesis directly and interactively with nERs.
