Quantification of Bifidobacterium spp., Escherichia coli and Clostridium difficile in faecal samples of breast-fed and formula-fed infants by real-time PCR
Quantification of Bifidobacterium spp., Escherichia coli and Clostridium difficile in faecal samples of breast-fed and formula-fed infants by real-time PCR
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DOI：
10.1016/j.femsle.2004.11.052
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发表时间：
2005-02-01
影响因子：
2.1
通讯作者：
Stobberingh, EE
中科院分区：
文献类型：
--
作者：
Penders, J;Vink, C;Stobberingh, EE
为了确定纯母乳喂养或配方奶喂养对婴儿肠道微生物区系组成和数量的影响，我们建立了实时定量PCR检测双歧杆菌的方法。和艰难梭菌。此外，我们还通过应用前面描述的实时荧光聚合酶链式反应(Real-Time-PCR)检测方法，监测了大肠杆菌的流行和数量。我们发现所有测试的100名婴儿都被双歧杆菌定植。50名母乳喂养的婴儿和50名配方奶粉喂养的婴儿的双歧杆菌计数相似，中位数分别为10.56log(10)和10.24log(10cfu g(-1)湿重粪便)。在14%的母乳喂养的婴儿和30%的配方奶粉喂养的婴儿中检测到艰难梭菌。此外，母乳喂养的婴儿艰难梭菌计数显著低于配方奶粉喂养的婴儿(中位数分别为3.28log(10)和7.43log(10)cfu g(-1)；p=0.03)。母乳喂养组和配方奶粉喂养组的大肠杆菌感染率分别为80%和94%。此外，母乳喂养婴儿的大肠埃希菌计数显著低于配方奶粉喂养婴儿(中位数分别为9.11log(10)和9.57log(10)cfu g(-1)；p=0.004)。结论：母乳喂养婴儿肠道微生物区系中艰难梭菌和大肠埃希菌的患病率和菌落数显著低于配方奶儿，而双歧杆菌的患病率和菌落数显著低于配方奶儿。在两组人中是相似的。(C)2004年欧洲微生物学会联合会。爱思唯尔出版，版权所有。
To determine the influence of either exclusive breast-feeding or formula feeding on both composition and quantity of the gut microbiota in infants, we have developed real-time, quantitative PCR assays for the detection of Bifidobacterium spp. and Clostridium difficile. Furthermore, we have monitored the prevalence and counts of Escherichia coli by applying a previously described real-time PCR assay. We found all 100 infants tested to be colonized by Bifidobacterium spp. The bifidobacterial counts were comparable between the 50 breast-fed and 50 formula-fed infants with median values of 10.56 log(10) and 10.24 log(10) CFU g(-1) wet weight faeces, respectively. C. difficile was detected in 14% of the breast-fed and 30% of the formula-fed infants. In addition, the C. difficile counts were significantly lower in breast-fed infants than in the formula-fed group (median values of 3.28 log(10) and 7.43 log(10) CFU g(-1), respectively; p = 0.03). The prevalence of E. coli in the breast-fed and formula-fed group was 80% and 94%, respectively. Also, the E coli counts in colonized infants was significantly lower in the breast-fed infants than in the formula-fed group (median values of 9.11 log(10) and 9.57 log(10) CFU g(-1), respectively; p = 0.004). We conclude that the prevalence and counts of C. difficile as well as E. coli are significantly lower in the gut microbiota of breast-fed infants than in that of formula-fed infants, whereas the prevalence and counts of Bifidobacterium spp. is similar among both groups. (C) 2004 Federation of European Microbiological Societies. Published by Elsevier B.V. All rights reserved.