Diazotroph diversity and nitrogen fixation in the coral Stylophora pistillata from the Great Barrier Reef
Diazotroph diversity and nitrogen fixation in the coral Stylophora pistillata from the Great Barrier Reef
复制标题
大堡礁珊瑚 Stylophora pistillata 的固氮生物多样性和固氮作用
DOI：
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发表时间：
2017
期刊：
影响因子：
--
通讯作者：
S. Noonan
中科院分区：
文献类型：
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作者：
M. Lesser;K. Morrow;Sabrina M. Pankey;S. Noonan
重氮菌，包括细菌和古生菌，能够固定氮(N_2)，存在于珊瑚的组织和粘液中，并能以氨(NH3)的形式用固定的氮(N)补充珊瑚全氮收支。从大堡礁苍鹭岛上采集的柱花鱼，在5和15 m处采集，并在实验室进行实验处理，结果表明，净光合作用速率、光系统II(PSII)荧光的稳态量子产率(∆Fv/Fm‘)和钙化程度与预期的照度有关。然而，氮的固定率在不同处理之间是不变的，而固定氮量对共生线虫有贡献。N需求依赖于辐照度。此外，共生菌和固氮菌群落在深度上也有显著差异，并恢复了新的簇V nifH基因系统型，这些新的基因系统型并不是已知的固定氮。用PICRUST进行的功能分析也表明，浅层珊瑚富含与氮代谢有关的基因，并具有特定的氮气固定作用。珊瑚已经进化出一系列策略来从有机(例如异养摄食)和无机来源(例如固定氮气)中获取氮，以维持蛋白质合成等关键途径，从而在氮有限的生境中取得生态上的成功。
Diazotrophs, both Bacteria and Archaea, capable of fixing nitrogen (N2), are present in the tissues and mucous, of corals and can supplement the coral holobiont nitrogen budget with fixed nitrogen (N) in the form of ammonia (NH3). Stylophora pistillata from Heron Island on the Great Barrier Reef collected at 5 and 15 m, and experimentally manipulated in the laboratory, showed that the rates of net photosynthesis, steady state quantum yields of photosystem II (PSII) fluorescence (∆Fv/Fm′) and calcification varied based on irradiance as expected. Rates of N2 fixation were, however, invariant across treatments while the amount of fixed N contributing to Symbiodinium spp. N demand is irradiance dependent. Additionally, both the Symbiodinium and diazotrophic communities are significantly different based on depth, and novel Cluster V nifH gene phylotypes, which are not known to fix nitrogen, were recovered. A functional analysis using PICRUSt also showed that shallow corals were enriched in genes involved in nitrogen metabolism, and N2 fixation specifically. Corals have evolved a number of strategies to derive nitrogen from organic (e.g., heterotrophic feeding) and inorganic sources (e.g., N2 fixation) to maintain critical pathways such as protein synthesis to succeed ecologically in nitrogen-limited habitats.
影响因子：
2.6
作者：
Cardini, Ulisse;Bednarz, Vanessa N.;Foster, Rachel A.;Wild, Christian
通讯作者：
Wild, Christian
影响因子：
64.8
作者：
Grosskopf, Tobias;Mohr, Wiebke;LaRoche, Julie
通讯作者：
LaRoche, Julie