Heat-shock protein 72 expression in excitotoxic versus penetrating injuries of the rodent cerebral cortex.
Heat-shock protein 72 expression in excitotoxic versus penetrating injuries of the rodent cerebral cortex.
复制标题
啮齿类动物大脑皮层兴奋性毒性损伤与穿透性损伤中热休克蛋白 72 的表达。
DOI：
10.1089/neu.1998.15.421
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
Walker,PD
中科院分区：
文献类型：
--
作者：
Dutcher,SA;Underwood,BD;Michael,DB;Diaz,FG;Walker,PD
The induction of heat shock protein 72 (hsp72) has been described in various experimental models of brain injury. The present study examinedhsp72 expression patterns within the rodent cerebral cortex in experimental paradigms designed to mimic two mechanisms of damage produced by penetration of the cerebral cortex: (1) tissue tearing from the missile track and (2) diffuse excitotoxicity during temporary cavitation and shock wave formation. Adult male Spaque-Dawley rats received controlled penetration (stab) or injection of the NMDA receptor excitotoxin, quinolinic acid (QA), into the frontal cortex and were killed 1–24 h later. Tissue from the lesioned, sham-operated, or contralateral uninjected cortex was processed for Western and immunocytochemical analyses ofhsp72 protein expression. By 12 h, both controlled penetration and excitotoxic brain injuries produced significant increases inhsp72 immunoreactivity, which decreased toward control levels at 24 h. However, the severity and regional distribution ofhsp72 expression varied between the two models. Specifically, the controlled penetration injury produced manyhsp72-expressing cells near the needle track, while immunoreactive cells within the QA-injected cortex were found in the periphery of the lesion site. Morphological assessment of brain sections subjected to dual-labeling procedures demonstrated that cells expressinghsp72 were primarily neuronal in both models of injury. These results suggest that although controlled penetration and diffuse excitotoxicity may induce similar temporal and cellular patterns ofhsp72 expression, the spatial location ofhsp72-immunoreactive cells may differ between the two models.
