In vivo label-free structural and biochemical imaging of coronary arteries using an integrated ultrasound and multispectral fluorescence lifetime catheter system
In vivo label-free structural and biochemical imaging of coronary arteries using an integrated ultrasound and multispectral fluorescence lifetime catheter system
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DOI：
10.1038/s41598-017-08056-0
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发表时间：
2017-08-21
影响因子：
4.6
通讯作者：
Marcu, Laura
中科院分区：
文献类型：
--
作者：
Bec, Julien;Phipps, Jennifer E.;Marcu, Laura
现有的临床血管内成像模式不能准确检测冠状动脉中的关键斑块病理生理学。这项研究报告了第一个血管内导管结合血管内超声（IVUS）与多光谱荧光寿命成像（FLIm），使无标记的同时评估冠状动脉血管的形态和生化特征在体内。基于40 MHz临床IVUS导管构建了带光纤通道的3.7 Fr导管。在猪冠状动脉中证明了使用右旋糖酐40溶液冲洗在体内安全采集共配准FLIm-IVUS数据的能力。来自动脉壁的FLIm参数与健康动脉壁中存在的荧光团（胶原蛋白、弹性蛋白）的发射一致。此外，动脉粥样硬化病变的结构和生化特征是在离体人冠状动脉样本中获得的，并与组织学结果相证实。目前的结果表明，FLIm参数与前200 μ m内膜中结构蛋白（如胶原蛋白、弹性蛋白）和脂质（如泡沫细胞、细胞外脂质）的量相关，提供了重要的生化信息，可以补充IVUS数据，用于斑块病理生理学的综合评估。本文评价的独特FLIm-IVUS系统有可能全面了解动脉粥样硬化病变的形成、诊断和治疗反应。
Existing clinical intravascular imaging modalities are not capable of accurate detection of critical plaque pathophysiology in the coronary arteries. This study reports the first intravascular catheter combining intravascular ultrasound (IVUS) with multispectral fluorescence lifetime imaging (FLIm) that enables label-free simultaneous assessment of morphological and biochemical features of coronary vessels in vivo. A 3.7 Fr catheter with a fiber-optic channel was constructed based on a 40 MHz clinical IVUS catheter. The ability to safely acquire co-registered FLIm-IVUS data in vivo using Dextran40 solution flushing was demonstrated in swine coronary arteries. FLIm parameters from the arterial wall were consistent with the emission of fluorophores present in healthy arterial wall (collagen, elastin). Additionally, structural and biochemical features from atherosclerotic lesions were acquired in ex vivo human coronary samples and corroborated with histological findings. Current results show that FLIm parameters linked to the amount of structural proteins (e.g. collagen, elastin) and lipids (e.g. foam cells, extracellular lipids) in the first 200 mu m of the intima provide important biochemical information that can supplement IVUS data for a comprehensive assessment of plaques pathophysiology. The unique FLIm-IVUS system evaluated here has the potential to provide a comprehensive insight into atherosclerotic lesion formation, diagnostics and response to therapy.