Serological relationship between a novel ovine pestivirus and classical swine fever virus
Serological relationship between a novel ovine pestivirus and classical swine fever virus
复制标题
DOI：
10.1111/tbed.13480
复制
发表时间：
2020-01-24
影响因子：
4.3
通讯作者：
De Mia, Gian Mario
中科院分区：
文献类型：
--
作者：
Casciari, Cristina;Sozzi, Enrica;De Mia, Gian Mario
瘟病毒属包括全球分布的黄病毒科成员，其导致牲畜的严重损失。该属最常见的种属是牛病毒性腹泻病毒1型（BVDV-1）和2型（BVDV-2）、猪瘟病毒（CSFV）和边界病病毒（BDV）。最近，在位于意大利布雷西亚省的一个农场的流产羔羊胎儿中反复检测到一种新的绵羊瘟病毒。该分离株的全基因组特征表明，它与已知的瘟病毒种高度不同，并且与CSFV在遗传上密切相关。本研究的目的是通过使用交叉中和试验进行抗原表征，确定已鉴别的新型瘟病毒与BVDV、BDV和CSFV选定毒株（可获得同源血清）之间的血清学相关性。血清学相关性表示为抗原相似系数（R）。抗绵羊瘟病毒的田间和特异性抗血清中和了CSFV参比毒株Diepholz，滴度显著高于BDV和BVDV毒株的特异性滴度。此外，计算的R值清楚地表明，与基因组分析的结果一致，新的绵羊瘟病毒在抗原性上与CSFV比与反刍动物瘟病毒更相关。这将对CSFV血清学产生严重后果，从而影响CSFV监测和猪健康管理。
The genus Pestivirus comprises globally distributed members of the family Flaviviridae, which cause severe losses in livestock. The most common species of the genus are bovine viral diarrhoea virus type 1 (BVDV-1) and type 2 (BVDV-2), classical swine fever virus (CSFV) and border disease virus (BDV). Recently, a novel ovine pestivirus was repeatedly detected in aborted lamb foetuses on a farm located in the Brescia Province (Italy). Complete genome characterization of this isolate showed that it was highly divergent from known pestivirus species and that it was genetically closely related to CSFV. The aim of this study was to determine the serological relatedness between the identified novel pestivirus and BVDV, BDV and CSFV selected strains for which homologous serum was available, by antigenic characterization performed using cross-neutralization assays. The serological relatedness was expressed as the coefficient of antigenic similarity (R). Both field and specific antisera raised against the ovine pestivirus neutralized the CSFV reference strain Diepholz with titres significantly higher than those specific for the BDV and BVDV strains. Furthermore, the calculated R values clearly indicated that the novel ovine pestivirus is antigenically more related to CSFV than to ruminant pestiviruses, in agreement with the results of the genomic analysis. This would have severe consequences on CSFV serology in the event of a switch to porcine hosts with implications for CSFV surveillance and porcine health management.