Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation.
Interaction between cytochalasin B-treated malarial parasites and erythrocytes. Attachment and junction formation.
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DOI：
10.1084/jem.149.1.172
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发表时间：
1979-01-01
期刊：
影响因子：
--
通讯作者：
Shiroishi T
中科院分区：
文献类型：
--
作者：
Miller LH;Aikawa M;Johnson JG;Shiroishi T
我们以前已经证明，疟疾裂殖子入侵红细胞(RBC)遵循一系列序列：识别和附着在顶端，与RBC的广泛变形有关，连接形成，连接围绕裂殖子移动，使裂殖子进入内陷的红细胞膜，以及膜的密封。在本文中，我们描述了一种在序列的早期阶段阻止入侵的方法。细胞松弛素处理的裂殖子特异性地附着于宿主红细胞，最常见的是通过含有与入侵相关的特殊细胞器的顶端区域。然后，寄生虫在顶端区域和红细胞之间形成一个连接。细胞松弛素阻止这一连接的运动，这是侵袭的后一步。细胞松弛素处理的(诺氏疟原虫)裂殖子附着在Duffy阴性的人红细胞上，尽管这些红细胞抵抗寄生虫的入侵。然而，与这些红细胞的附着不同于敏感的红细胞，因为没有连接形成。因此，Duffy相关抗原似乎参与了连接的形成，而不是最初的附着。
We have previously demonstrated that invasion of erythrocytes (RBCs) by malaria merozoites follows a sequence: recognition and attachment in an apical orientation associated with widespread deformation of the RBC, junction formation, movement of the junction around the merozoite that brings the merozoite into the invaginated RBC membrane, and sealing of the membrane. In the present paper, we describe a method for blocking invasion at an early stage in the sequence. Cytochalasin-treated merozoites attach specifically to host RBCs, most frequently by the apical region that contains specialized organelles (rhoptries) associated with invasion. The parasite then forms a junction between the apical region and the RBC. Cytochalasin blocks movement of this junction, a later step in invasion. Cytochalasin-treated (Plasmodium knowlesi) merozoites attach to Duffy-negative human RBCs, although these RBCs are resistant to invasion by the parasite. The attachment with these RBCs, however, differs from susceptible RBCs in that there is no junction formation. Therefore the Duffy associated antigen appears to be involved in junction formation, not initial attachment.