Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with high molar fractions of 3-hydroxyvalerate by a threonine-overproducing mutant of Alcaligenes sp SH-69
Production of poly(3-hydroxybutyrate-co-3-hydroxyvalerate) with high molar fractions of 3-hydroxyvalerate by a threonine-overproducing mutant of Alcaligenes sp SH-69
复制标题
DOI：
10.1023/a:1023437013044
复制
发表时间：
2003-05-01
影响因子：
2.7
通讯作者：
Rhee, YH
中科院分区：
文献类型：
--
作者：
Choi, GG;Kim, MW;Rhee, YH
分离了一株高产苏氨酸突变体Alcaligenes sp. SH-69，并对其产聚（3-羟基丁酸-co-3-羟戊酸）poly（3HB-co-3HV）的能力进行了研究。以葡萄糖为唯一碳源的聚（3HB-co-3HV）的3HV分数超过22 mol%，在相同的培养条件下，比野生型的3HV分数高约6倍。此外，在葡萄糖培养基中加入较低浓度（10 mM）的丙酸、戊酸或乙酰丙酸，可大大提高共聚酯中3HV的摩尔分数，达到38-77 mol%。结果表明，对提供聚羟基烷酸单体的生物合成途径进行代谢工程，如苏氨酸生物合成途径，可以产生新的聚（3HB-co-3HV）产生菌株。
A threonine overproducing mutant of Alcaligenes sp. SH-69 was isolated and its ability to produce poly(3-hydroxybutyrate-co-3-hydroxyvalerate), poly(3HB-co-3HV), was investigated. The 3HV fraction in poly(3HB-co-3HV) produced from glucose as the sole carbon source exceeded 22 mol%, which is approximately six times higher than that achieved by the wild type under the same culture conditions. Furthermore, the addition of a relatively low concentration (10 mM) of propionic acid, valeric acid or levulinic acid to the glucose medium greatly increased the molar fraction of 3HV in the copolyester, to 38-77 mol%. The results suggest that metabolic engineering of the biosynthetic pathways supplying polyhydroxyalkanoate monomers, such as the threonine biosynthetic pathway, can lead to new poly(3HB-co-3HV)-producing strains.