MULTICOPY SUPPRESSION OF THE CDC24 BUDDING DEFECT IN YEAST BY CDC42 AND 3 NEWLY IDENTIFIED GENES INCLUDING THE RAS-RELATED GENE RSR1
MULTICOPY SUPPRESSION OF THE CDC24 BUDDING DEFECT IN YEAST BY CDC42 AND 3 NEWLY IDENTIFIED GENES INCLUDING THE RAS-RELATED GENE RSR1
复制标题
DOI：
10.1073/pnas.86.24.9976
复制
发表时间：
1989-12-01
影响因子：
11.1
通讯作者：
PRINGLE, JR
中科院分区：
文献类型：
--
作者：
BENDER, A;PRINGLE, JR
CDC24、CDC42和CDV43基因是酿酒酵母细胞极性确定和分泌定位所必需的；这些基因缺失的突变株不能形成芽并表现出细胞表面的各向同性扩张。为了确定可能参与这些过程的其他基因，我们筛选了酵母基因组DNA文库中的异源基因，当从质粒中过度表达时，可以抑制温度敏感的cdc24突变。我们确定了四个这样的基因。其中一个被证明是cdc42，它以前被证明是Rho(ras同源)基因家族的成员，第二个是新发现的ras相关基因，我们将其命名为RSR1。RSR1定位于第七号染色体右臂的CDC62和ADE3之间；其预测产物为.apprxeq。与ras家族的其他蛋白有50%的同源性。RSR1的缺失是非致命性的，但会扰乱正常的芽位选择模式。虽然CDC42和RSR1都可以抑制CDC24，而且两者似乎都编码GTP结合蛋白，但这些基因本身似乎不能在功能上互换。然而，另一个因其抑制cdc24的能力而被分离出来的基因也可以抑制cdc42。该基因被命名为MSB1，位于第15号染色体右臂上的ADE9和HIS3之间。
Genes CDC24, CDC42, and CDV43 are required for the establishment of cell polarity and the localization of secretion in Saccharomyces cerevisiae; mutants defective in these genes fail to form buds and display isotropic expansion of the cell surface. To identify other genes that may be involved in these processes, we screened yeast genomic DNA libraries for heterologous genes that, when overexpressed from a plasmid, can suppress a temperature-sensitive cdc24 mutation. We identified four such genes. One of these proved to be CDC42, which has previously been shown to be a member of the rho (ras-homologous) family of genes, and a second is a newly identified ras-related gene that we named RSR1. RSR1 maps between CDC62 and ADE3 on the right arm of chromosome VII; its predicted product is .apprxeq. 50% identical to other proteins in the ras family. Deletion of RSR1 is nonlethal but disrupts the normal pattern of bud site selection. Although both CDC42 and RSR1 can suppress cdc24 and both appear to encode GTP-binding proteins, these genes do not themselves appear to be functionally interchangeable. However, one of the other genes that was isolated by virtue of its ability to suppress cdc24 can also suppress cdc42. This gene, named MSB1, maps between ADE9 and HIS3 on the right arm of chromosome XV.