Dependence of the community effect of cultured cardiomyocytes on the cell network pattern
Dependence of the community effect of cultured cardiomyocytes on the cell network pattern
复制标题
DOI：
10.1016/j.bbrc.2007.03.005
复制
发表时间：
2007-05-04
影响因子：
3.1
通讯作者：
Yasuda, Kenji
中科院分区：
文献类型：
--
作者：
Kaneko, Tomoyuki;Kojima, Kensuke;Yasuda, Kenji
为了阐明群体效应在心肌细胞中的作用，我们开发了一种基于芯片的单细胞培养系统，该系统可以在单细胞水平上测量培养的心肌细胞在细胞网络形成前后的心率变化和心率波动的动态。我们通过在不同形状的微室中培养心肌细胞，考察了群体效应对细胞网络格局的依赖性，并探讨了网络格局与心跳稳定性的关系。我们发现，无论蜂窝网络模式如何，随着蜂窝群落规模的增加，节拍率波动趋于减小。这表明，芯片上基于单细胞的心肌细胞群落可能能够足够准确地模拟心脏组织，从而用于药物筛选等实际应用。(c) 2007爱思唯尔公司版权所有。
To elucidate the role of the community effect in cardiomyocytes, we developed an on-chip single-cell-based culture system with which the dynamics of the change of beat rate and beat rate fluctuation of cultured cardiomyocytes can be measured at the single-cell level before and after the formation of a cell network. We examined the dependence of the community effect on cell network pattern by culturing cardiomyocytes in differently shaped microchambers and investigated the relation between the network pattern and the stability of the beat rate. We found that beat rate fluctuation tends to decrease as cell-community size increases, irrespective of cell network pattern. This indicates that on-chip single-cell-based cardiomyocyte communities might be able to model a heart tissue accurately enough to be used in practical applications such as drug screening. (c) 2007 Elsevier Inc. All rights reserved.