Microsatellite markers reveal the below ground distribution of genets in two species of Rhizopogon forming tuberculate ectomycorrhizas on Douglas fir
Microsatellite markers reveal the below ground distribution of genets in two species of Rhizopogon forming tuberculate ectomycorrhizas on Douglas fir
复制标题
DOI：
10.1046/j.1469-8137.2003.00915.x
复制
发表时间：
2003-11
期刊：
影响因子：
9.4
通讯作者：
A. Kretzer;S. Dunham;R. Molina;J. Spatafora
中科院分区：
文献类型：
--
作者：
A. Kretzer;S. Dunham;R. Molina;J. Spatafora
我们已经开发了两个姐妹种的微卫星标记，R. vesiculosus和R. vinicolor（牛肝菌目，担子菌门），并使用选定的标记调查外生菌根样品的基因大小和分布。这两个物种在花旗松（花旗松）上形成具有瘤状形态的外生菌根。·在位于美国俄勒冈州海岸山脉玛丽峰和俄勒冈州喀斯喀特山脉米尔克里克的两个10 × 10 m核心地块中对外生菌根进行了采样和绘图;从玛丽峰核心地块周围的更大区域获得了额外的样本。·在新发现的微卫星位点上，R的基因多样性在0.00 ~ 0.68之间。vesiculosus的含量为0.00 ~ 0.43; vinicolor。这两个类群似乎在Hardy-Weinberg和连锁平衡。同一种属的根瘤外生菌根间的最大距离为13.4m; vesiculosus，而R. vinicolor。·据我们所知，这是第一项使用多个共显性标记区分真菌基因与外生菌根的研究。
Summary • We have developed microsatellite markers for two sister species of Rhizopogon, R. vesiculosus and R. vinicolor (Boletales, Basidiomycota), and used selected markers to investigate genet size and distribution from ectomycorrhizal samples. Both species form ectomycorrhizas with tuberculate morphology on Douglas-fir (Pseudotsuga menziesii). • Tuberculate ectomycorrhizas were sampled and mapped in two 10 × 10 m core plots located at Mary's Peak in the Oregon Coast Range and at Mill Creek in the Oregon Cascade Mountains, USA; additional samples were obtained from a larger area surrounding the Mary's Peak core plot. • Gene diversities at the newly described microsatellite loci ranged from 0.00 to 0.68 in R. vesiculosus, and from 0.00 to 0.43 in R. vinicolor. Both taxa appeared to be in Hardy–Weinberg and linkage equilibrium. The largest distance observed between tuberculate ectomycorrhizas of the same genet was 13.4 m for R. vesiculosus, but only 2 m for R. vinicolor. • This is to our knowledge the first study to differentiate fungal genets from ectomycorrhizas with great confidence using multiple codominant markers.